FastQCFastQC Report
Sat 16 Sept 2023
SRR15513198_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513198_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34631016
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN30543718.819755677973756No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN22542406.509309458319097No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN8937502.580779033453711No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN7543482.1782439186883806No Hit
AGACGTGGCGACCCGCTGAATTTAAGCNNNNNNNN5566071.607249986543854No Hit
AGACGTGGCGACCCGCTGAATTTAAGCATNNNNNN5537051.598870215069636No Hit
ATACCACCCTGAACGCGCCCGATCTCGTCNNNNNN4360631.2591689484362802No Hit
AAGGCTAAATACCGGCACGAGACCGATNNNNNNNN4267511.2322797575445088No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3914811.1304346369739773No Hit
AAGAGGGCGTGAAACCGTTAAGAGGTNNNNNNNNN3776691.0905513138857954No Hit
AGATCAGACGTGGCGACCCGCTGAATTTNNNNNNN3590561.0368046955365098No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN3554201.0263054367218103No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGNNN2596140.7496574746753026No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAAGCNNN1903510.549654679493088No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTNNNN1853350.5351705534714892No Hit
ATCCGGTAAAGCGAATGATTAGAGGTNNNNNNNNN1804060.5209376473390211No Hit
ATCTAAGGCTAAATACCGGCACGAGACCGATNNNN1660590.47950946631193264No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN1650770.4766738579081826No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN1590170.45917509321701677No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN1454450.41998479051264337No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGT1405950.4059800035898456No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN1330480.38418740010399927No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTNNNNNNNN1317360.3803988886725125No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN1278280.3691142067561633No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN1270620.366902316697841No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN1229020.3548899633784929No Hit
TTTATCCGGTAAAGCGAATGATTAGAGGTNNNNNN1173650.3389014056070431No Hit
ATACCACCCTGAACGCGCCCGATCTCGTNNNNNNN1142860.3300105315997659No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN1127170.32547991084061756No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGANN1119260.32319583115898187No Hit
CGATGAAGAACGCAGCTAGCTGCGAGAATTNNNNN1100820.3178711245433862No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN1074900.3103865043982538No Hit
ACCACCCTGAACGCGCCCGATCTCGTCNNNNNNNN1022410.29522957108737435No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACNN978180.28245778293076934No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGNNNNNNN975410.281657921904457No Hit
AGCTGCGAGAATTAATGTGAATTGCAGNNNNNNNN935910.2702519614209413No Hit
AGGAGAGGAGCGAGCGACCAAAGGAACCNNNNNNN910760.2629896853156142No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN892130.25761011458630034No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN840100.2425860101823175No Hit
AAACCCTTGTGTCGAGGGCTGACTTTCAATNNNNN820240.23685126650630173No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN814700.23525154445367702No Hit
AGACAGCAGGACGGTGGCCATGGAAGTNNNNNNNN761290.21982895332900426No Hit
ACGGACCAAGGAGTCTAACACGTGCGCGAGTNNNN750510.21671613677173088No Hit
AGCGACGGGCGGTGTGTACAAAGGGCNNNNNNNNN745420.21524635604107026No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTNNNN741980.21425302682427796No Hit
AAATGGGTAAGAAGCCCGGCTCGCTGGCGTNNNNN709110.2047615351510334No Hit
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTNNNNN699680.20203854255965234No Hit
ATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNN697560.20142637455395476No Hit
AATCATGGCCTCAGTTCCGAAAACCAACNNNNNNN687110.19840884829945504No Hit
ACTCGGCTCGTGCGTCGATGAAGAACGCNNNNNNN680350.19645684088506093No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCNNNNNNN676700.19540287238468546No Hit
ACGTGTTAGGACCCGAAAGATGGTGAACTNNNNNN665960.1923016061671422No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNN660730.19079139924742605No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN633190.18283899034322296No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN618640.17863755426638364No Hit
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCNNNNN612970.17700029360963593No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTNNNN588640.16997479946877678No Hit
AGCGCCGAATCCCCGCCCCGCGGCGGGGCGCGGGAC588410.16990838501532846No Hit
ACTCCCGACCCGGGGAGGTAGTGACGAAAAATNNN583620.1685252318326439No Hit
ATGGCCTCAGTTCCGAAAACCAACAAAATNNNNNN582130.1680949816776961No Hit
AAGCAGAACTGGCGCTGCGGGATGAACCNNNNNNN575640.16622093905648047No Hit
GCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTN570770.16481468519433562No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCNNN568130.16405236277214622No Hit
AGCGGTGGATCACTCGGCTCGTGCGTCGATNNNNN566620.16361633744733334No Hit
TTTTATCCGGTAAAGCGAATGATTAGAGGTNNNNN562930.1625508186072277No Hit
AAATACCGGCACGAGACCGATAGTCAACNNNNNNN553120.15971809778841026No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTNNN529350.1528543084037731No Hit
AAGAGCATCGAGGGGGCGCCGAGAGGCNNNNNNNN515350.1488116894982232No Hit
AGACGTGGCGACCCGCTGAATTTAAGCATATTNNN514170.148470954476184No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTNNN514090.1484478537967237No Hit
AATCGGTAGTAGCGACGGGCGGTGTGTACNNNNNN512870.1480955684349544No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGANN499220.14415401500204325No Hit
AATCGGTAGTAGCGACGGGCGGTGTGTNNNNNNNN494650.1428343886878745No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCNNNNNN490010.14149454927917796No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNN475190.13721514840916016No Hit
ACAAAGGGCAGGGACTTAATCAACGCNNNNNNNNN474990.13715739671050944No Hit
CAACTTCTTAGAGGGACAAGTGGCGTTCNNNNNNN474150.13691483957617645No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN473900.13684264995286305No Hit
ACTTCGAACGCACTTGCGGCCCCGGGNNNNNNNNN466530.1347144998575843No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN463260.13377025958464517No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCNNNNNN461570.13328225773104663No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN461100.13314654123921746No Hit
AGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTNNNN455080.13140821510983103No Hit
AGCTGGTTCCCTCCGAAGTTTCCCTCAGGATNNNN450940.1302127549477613No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTNNNN450470.1300770384559321No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGANNN445780.12872276112257292No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCNNNNN439610.12694112121919843No Hit
AGATCAGACGTGGCGACCCGCTGAATTNNNNNNNN435290.125693684528343No Hit
GTCGATGAAGAACGCAGCTAGCTGCGAGAATTNNN420090.1213045554308889No Hit
AAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGC419540.12114573825959944No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCNNNNNNN418910.12096382040884968No Hit
TTATCCGGTAAAGCGAATGATTAGAGGTNNNNNNN416090.12014952145787464No Hit
AAGGGCCAGCGAGAGCTCACCGGACGCCGCCNNNN408520.11796361966394517No Hit
GAGAGATGGGCGAGCGCCGTTCCGAAGGGACNNNN402550.11623973145922142No Hit
GCCGAATCCCCGCCCCGCGGCGGGGCGCGGGACNN402340.11617909217563817No Hit
AAGCAGAACTGGCGCTGCGGGATGAACNNNNNNNN400320.11559580001926596No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGNNN397460.11476995072856076No Hit
AGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCN395340.11415778272286323No Hit
AGCTGCGAGAATTAATGTGAATTGCNNNNNNNNNN383670.11078797110659416No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCC382350.11040680989549945No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGTNNNNNN375920.10855009278387905No Hit
GTCTAGGGGTATGATTCTCGCTTNNNNNNNNNNNN369420.10667316257773089No Hit
GTTTTATCCGGTAAAGCGAATGATTAGAGGTNNNN362470.10466629104961864No Hit
AAGGGCCAGCGAGAGCTCACCGGACGCCGCNNNNN358590.10354590809579484No Hit
AGCTCTTTGGACACTAGGAAAAAACCTTGTNNNNN358390.10348815639714411No Hit
AACCTATTCTCAAACTTTAAATGGGTNNNNNNNNN358390.10348815639714411No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNN349970.10105680988394912No Hit
AGTAGCGACGGGCGGTGTGTACAAAGGGCNNNNNN346640.10009524410141475No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)