FastQCFastQC Report
Sat 16 Sept 2023
SRR15513199_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513199_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28749480
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACGTGGCGACCCGCTGAATTTAAGCATNNNNNN9800423.4089033958179416No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN7252372.5226091045820653No Hit
AGATCAGACGTGGCGACCCGCTGAATTTNNNNNNN6354742.210384326951305No Hit
AGACGTGGCGACCCGCTGAATTTAAGCNNNNNNNN5985242.081860263211717No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN5392371.8756408811568068No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN3814821.3269179129500777No Hit
AAGAGGGCGTGAAACCGTTAAGAGGTNNNNNNNNN3698181.2863467443585066No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN3103091.0793551744240244No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3019251.0501929078369416No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAAGCNNN2660640.9254567386957955No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCNNNNNN2144980.7460934945605973No Hit
AGGAGAGGAGCGAGCGACCAAAGGAACCNNNNNNN2045810.7115989576159291No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGT2032890.7071049632897708No Hit
AAATGGGTAAGAAGCCCGGCTCGCTGGCGTNNNNN1743170.6063309666818322No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTNNNN1603880.5578813947243567No Hit
AGCGGTGGATCACTCGGCTCGTGCGTCGATNNNNN1478900.5144093040987177No Hit
AAGAGCATCGAGGGGGCGCCGAGAGGCNNNNNNNN1379180.47972345934604727No Hit
AAGGCTAAATACCGGCACGAGACCGATNNNNNNNN1318150.45849524930537877No Hit
ACGTGTTAGGACCCGAAAGATGGTGAACTNNNNNN1305240.4540047333030024No Hit
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTNNNNN1255650.4367557256687773No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCNNN1247530.43393132675791No Hit
CGATGAAGAACGCAGCTAGCTGCGAGAATTNNNNN1191120.41431010230445914No Hit
AGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTNNNN1161790.4041081786522747No Hit
GAAGCCAGAGGAAACTCTGGTGGAGGTCCGTNNNN1137650.39571150504287383No Hit
AGACGTGGCGACCCGCTGAATTTAAGCATATTNNN998550.34732802123725365No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGNNN963040.3349764934878822No Hit
ATCTAAGGCTAAATACCGGCACGAGACCGATNNNN949390.33022858152564843No Hit
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCNNNNN934510.3250528357382464No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN916950.3189448991773069No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCC916220.3186909815412314No Hit
AGCGACGGGCGGTGTGTACAAAGGGCNNNNNNNNN890490.30974125445051526No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGNNNNNNN878840.30568900724465276No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN863100.30021412561201105No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGTNNNNNN857780.2983636573600636No Hit
ATGGCCTCAGTTCCGAAAACCAACAAAATNNNNNN847510.2947914188360972No Hit
AGCTGCGAGAATTAATGTGAATTGCAGNNNNNNNN835670.29067308347837945No Hit
GACGGCGCGACCCGCCCGGGGCGCACTGGGGACNN835240.2905235155557596No Hit
GTCGATGAAGAACGCAGCTAGCTGCGAGAATTNNN832330.2895113233352395No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN811410.2822346699835962No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTNNNN794630.27639804267764145No Hit
CGGGAGGGAGGAAGACGAACGGAAGGACNNNNNNN793250.2759180339957453No Hit
AAGGGCCAGCGAGAGCTCACCGGACGCCGCCNNNN766880.26674569418299043No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN757920.2636291160744473No Hit
GAACGGGGAGGAGGCGGGAACCGAAGAAGCNNNNN750560.2610690697710011No Hit
ACGGACCAAGGAGTCTAACACGTGCGCGAGTNNNN749580.26072819404037917No Hit
GCGAGTGCCTAGTGGGCCACTTTTGGTAAGCNNNN747150.2598829613613881No Hit
AGCTAAGAGCATCGAGGGGGCGCCGAGAGGCNNNN745400.2592742546995633No Hit
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCNNNN742350.2582133659460971No Hit
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACNNNN739030.2570585624505209No Hit
GCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTN730950.254248076834781No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGANN715630.24891928480097725No Hit
AATCGGTAGTAGCGACGGGCGGTGTGTNNNNNNNN711570.24750708534554364No Hit
GAACGGAAGGACGGACGGCGCCGGACGCGCNNNNN708790.2465401113341876No Hit
AATCATGGCCTCAGTTCCGAAAACCAACNNNNNNN705720.24547226593315774No Hit
AAACCCTTGTGTCGAGGGCTGACTTTCAATNNNNN698000.24278699997356476No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTNNNNNNNN685760.2385295316645727No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN684700.23816082934369595No Hit
AATCGGTAGTAGCGACGGGCGGTGTGTACNNNNNN649330.22585799812727048No Hit
AGTGCGGTAACGCGACCGATCCCGGAGAAGCNNNN648970.22573277847112364No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTNNN646740.2249571122677697No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN641000.22296055441698423No Hit
GCGGAGGAGCGAGGAGGAAGGACGCGCNNNNNNNN636930.22154487663776876No Hit
AAGGGCCAGCGAGAGCTCACCGGACGCCGCNNNNN635080.22090138673812534No Hit
ACTCGGCTCGTGCGTCGATGAAGAACGCNNNNNNN618070.2149847579851879No Hit
ACGAGCGGCGCCGGGGAGCGGGTCTTCCGTNNNNN590060.2052419730722086No Hit
AGGAGAGGAGCGAGCGACCAAAGGAACNNNNNNNN589110.20491153231293224No Hit
ACGATCCTTCTGACCTTTTGGGTTTTAAGCNNNNN579160.20145060014998534No Hit
GAGGAGGAAGGACGCGCGAGGGCCGGGACCCCNNN569470.19808010440536664No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCNNNNNNN562310.19558962457755757No Hit
AGTAACGGCGAGTGAACAGGGAAGAGCCCNNNNNN560110.1948243933455492No Hit
ATCCGGTAAAGCGAATGATTAGAGGTNNNNNNNNN559620.19465395548023826No Hit
AGGCGGGGGACCGGCTATCCGAGGCCAACCNNNNN550570.19150607245765838No Hit
ATGAGAGGTGTAGAATAAGTGGGAGGCCCNNNNNN546510.19009387300222474No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTNNNN541040.1881912298935494No Hit
AGATCAGACGTGGCGACCCGCTGAATTNNNNNNNN526590.18316505202876712No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN518490.1803476097654636No Hit
GTGGCCCCGAGAGAACCTCCCCCGGGCCCGACGGCGC517720.18007977883426066No Hit
GGAGGTGGAGCACGAGCGCACGTGTTNNNNNNNNN514420.1789319319862481No Hit
GAAAGCCGCCGTGGCGCAATGAAGGTGAAGGCCNN510960.17772843195772584No Hit
AGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCN508860.17699798396353605No Hit
AGGACGAAGGGCACTCCGCACCGGACCCNNNNNNN502060.1746327237918738No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNN495430.17232659512450313No Hit
GTGGCGTACGGAAGACCCGCTCCCCGGCGCNNNNN493510.17165875695838673No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATNNNNN484200.16842043751747857No Hit
AGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTT482450.16781173085565373No Hit
AAAGCATCGCGAAGGCCCGCGGCGGGTGTTNNNNN482110.16769346784707062No Hit
AAATACCGGCACGAGACCGATAGTCAACNNNNNNN480030.16696997650044454No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCNNNNNNN464790.16166901105689563No Hit
AGACAGCAGGACGGTGGCCATGGAAGTNNNNNNNN464760.16165857608555007No Hit
AGTACTTGGATGGGAGACCGCCTGGGAATNNNNNN463520.16122726393659992No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCAATNNN454180.15797850952434617No Hit
AGGAAGAGCCGACATCGAAGGATCAAAAAGCNNNN450740.15678196614338766No Hit
AGTGCGGTAACGCGACCGATCCCGGAGAAGCCGGC443480.15425670307776002No Hit
AGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGAC437930.1523262333788298No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCNNNNN434860.15125838797779995No Hit
ACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTNNN434160.15101490531307No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCNNNNNNN433350.15073316108673965No Hit
GGGAGGTGGAGCACGAGCGCACGTGTTNNNNNNNN431080.1499435815882583No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGNNNN414580.1442043473481955No Hit
AGTAGCGACGGGCGGTGTGTACAAAGGGCNNNNNN409940.14259040511341423No Hit
AGTAGCTGGTTCCCTCCGAAGTTTCCCTCNNNNNN405110.14091037472677767No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACNN401840.13977296285011068No Hit
ACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGC399250.1388720769906099No Hit
GAGACGGGCCGGTGGTGCGCCCTCGGCGGACTNNN394460.13720595989910078No Hit
AACGGCGAGTGAACAGGGAAGAGCCCNNNNNNNNN394010.13704943532891725No Hit
AGGCACGGCGACTACCATCGAAAGTTGATNNNNNN393250.13678508272149617No Hit
GAGAGATGGGCGAGCGCCGTTCCGAAGGGACNNNN391180.13606506969865195No Hit
ACTCGGCTCGTGCGTCGATGAAGAACGCAGCNNNN384540.1337554627074994No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCNNNNNN383370.13334849882502223No Hit
AATGATAGGAAGAGCCGACATCGAAGGATCNNNNN382630.13309110286516487No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACGGGCNN379800.13210673723489955No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTGCNN375870.13073975598863005No Hit
AAGGCGCCCGATGCCGACGCTCATCAGACCCCNNN369780.1286214568054796No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN367560.12784926892590753No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN367340.1277727458027067No Hit
GAGTGCCTAGTGGGCCACTTTTGGTAAGCNNNNNN366670.1275396981093223No Hit
ACTCCCGACCCGGGGAGGTAGTGACGAAAAATNNN366200.12737621689157508No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACNNNNNN350140.12179002889791397No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGC343220.11938302884086946No Hit
GAGCGGCGCCGGGGAGCGGGTCTTCCGTNNNNNNN339800.11819344210747464No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCGGTNNN336250.11695863716491568No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTNNNN327890.11405075848328386No Hit
ACAAAGGGCAGGGACTTAATCAACGCNNNNNNNNN325840.11333770210800334No Hit
AGCGGTGGATCACTCGGCTCGTGCGTCNNNNNNNN318730.11086461389910357No Hit
AGCTGGTTCCCTCCGAAGTTTCCCTCAGGATNNNN317710.11050982487335423No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATN317680.11049938990200865No Hit
AGAAAAGGTGTTGGTTGATATAGACAGCNNNNNNN315100.10960198236628976No Hit
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCNNN314300.10932371646374126No Hit
ACGCTCCCCGGACCCCGTCCCGGCCCGGAGCGGAC313120.10891327425748222No Hit
ACTTGGATGGGAGACCGCCTGGGAATNNNNNNNNN312030.1085341369652599No Hit
GCCGAATCCCCGCCCCGCGGCGGGGCGCGGGACNN311780.10844717887071348No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN299560.10419666720928517No Hit
ACAAGCTTGGGCTGCAGGTCGACCCGTGCNNNNNN299370.10413057905742991No Hit
AAGGCTAAATACCGGCACGAGACCGATAGTCNNNN298270.10374796344142573No Hit
AGAAGCAGGTCGTCTACGAATGGTTTAGCGCCNNN292620.10178271050467695No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)