FastQCFastQC Report
Sat 16 Sept 2023
SRR15513200_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513200_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55927471
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN19177833.4290536756078245No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN15743222.8149350790419256No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNN11694892.0910815008960446No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN10275291.8372527518721524No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9994341.7870180469987638No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN8010241.4322550004093695No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN7987841.4282498130480457No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNN7576511.3547027720956666No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN7068951.2639495177602436No Hit
ATTGATCATCGACACTTCGAACGCACNNNNNNNNN6097971.0903353738272914No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN5888221.0528314430666819No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN5221770.9336681789169404No Hit
CTAAACCATTCGTAGACGACCTGCTTNNNNNNNNN4512980.8069343954422684No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN3301610.590337796608039No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNN3063810.5478184414954146No Hit
TTGATCATCGACACTTCGAACGCACNNNNNNNNNN3030530.5418678774157337No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN2987270.5341328593241772No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN2860600.5114838824019059No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN2785130.4979896194483745No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNN2556340.4570812794306397No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN2212210.39554980056223177No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN2037370.36428788278304236No Hit
ATTGATCATCGACACTTCGAACGCANNNNNNNNNN1995920.35687649813452144No Hit
AGTGCGGTAACGCGACCGATCCCGGAGANNNNNNN1982150.3544143807253505No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNNN1836880.3284396678691228No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNNN1823070.3259703983396639No Hit
TTCACTGACCCGGTGAGGCGGGGNNNNNNNNNNNN1816790.32484751545443563No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNN1758270.3143839634729773No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN1683990.3011024760980163No Hit
TTTTCACTGACCCGGTGAGGCGGGGNNNNNNNNNN1663170.29737979748807164No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN1552730.277632793372688No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN1537110.2748398904001935No Hit
TTTCACTGACCCGGTGAGGCGGGGNNNNNNNNNNN1536890.27480055373860907No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNN1459450.26095404886088985No Hit
ATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNN1445640.2584847793314309No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN1409080.2519477413881275No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN1379120.246590803292357No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGNNN1332970.23833904450998689No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNN1322150.23640439597206173No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN1321330.23625777750615617No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGNN1320920.23618446827320333No Hit
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGNNNNN1307400.23376705161583292No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN1287040.23012662238920117No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNN1237570.2212812376229206No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN1215890.21740478842678226No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN1165130.2083287477812111No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN1163750.20808199963127244No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN1126280.2013822509514153No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNN1102390.19711064710936063No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN1092550.1953512255184934No Hit
CTCCCGGGGCTACGCCTGTCTGAGNNNNNNNNNNN995020.17791256822608698No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN994050.17773912930910105No Hit
AGACGTGGCGACCCGCTGAATTTAAGCATNNNNNN985280.17617102693593995No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN984700.1760673211917628No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTNN880550.15744498799167944No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGNNNNNNN832820.14891072045793025No Hit
TTGATCATCGACACTTCGAACGCANNNNNNNNNNN830250.14845119672942123No Hit
TTTGGTGACTCTAGATAACCTCGGGNNNNNNNNNN827930.14803637375271267No Hit
TGAAGGGCAGGGCGCCCTGGAATGGGTTNNNNNNN823650.14727109688188833No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN817070.14609457309449947No Hit
GTCTAGCGGTTAGGATTCCTGGNNNNNNNNNNNNN812980.1453632687950435No Hit
AAGGCTAAATACCGGCACGAGACCGATNNNNNNNN804330.14381662278274662No Hit
GTCTAGGGGTATGATTCTCGCTTNNNNNNNNNNNN801010.14322299679883613No Hit
AGACGTGGCGACCCGCTGAATTTAAGCNNNNNNNN776030.13875649767893133No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNN752550.1345582030698295No Hit
CGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNNN741980.13266825528370485No Hit
AGTGCGGTAACGCGACCGATCCCGGAGNNNNNNNN738460.13203886869835396No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN730770.13066387357297096No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN721800.12906001059836944No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCNNNNNNN706820.1263815415504842No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGNNNN702970.12569314997275668No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN672630.12026826673424942No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATG662030.11837295485790875No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTNNNNNNNN660790.11815123912897832No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTNNNNN658560.11775250842291796No Hit
CCAGGTGGCCCGGGTTCGACTCCCGGTATGGGNNN653880.11691571034921282No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNN642480.11487735606711055No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN641610.11472179745084486No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGANNN631970.11299813646141804No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNNN618530.11059502404462379No Hit
GCCGCGGCCCGGGTTCGATTCCCGGTCAGGGNNNN606520.10844759992812834No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCNNNNNN586620.10488942008480948No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGNNNN582500.10415275169513745No Hit
ATTGATCATCGACACTTCGAACGCNNNNNNNNNNN578770.10348581647827415No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCNNN577340.10323012817797536No Hit
ATCCGGTAAAGCGAATGATTAGAGGTNNNNNNNNN571370.10216267422497971No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC571040.10210366923260306No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNNN564500.1009342975655023No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN562500.10057669155109838No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)