FastQCFastQC Report
Sat 16 Sept 2023
SRR15513201_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513201_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25777818
Total Bases907.3 Mbp
Sequences flagged as poor quality0
Sequence length35-51
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8966423.4783471587858985No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNN7355072.853255461730702No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN6133012.379181201450022No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN3332201.292661776105332No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN3189011.2371140179514029No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNN3126071.2126976767389699No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN2847521.1046396556915716No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN2607511.0115324733846751No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNN2140670.8304310318274418No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN2033700.7889341138183225No Hit
ATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNN1824510.707782947338677No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN1693360.6569058715520453No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN1560380.6053188830800187No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGNN1375920.5337612361139333No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNN1105610.4288997618029579No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGANNNNNN950100.36857270076156173No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN932810.3618653836410824No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN924700.3587192678604528No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN878580.3408279164667855No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNN766250.2972516913572747No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN751970.29171204482861973No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN724260.2809624926361107No Hit
ATCGTATAGTGGTTAGTACTCTGCGNNNNNNNNNN679230.26349398541024693No Hit
AGTCCGCCCGGAGGATTCAACCCGNNNNNNNNNNN646740.2508901257662693No Hit
ATTGATCATCGACACTTCGAACGCANNNNNNNNNN621830.24122677877545726No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNNN610770.23693626822875388No Hit
TCGCTTCTGGCGCCAAGCGCCCGGNNNNNNNNNNN598840.23230825820866607No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN591500.22946084885850307No Hit
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGNNNNN533320.20689105648895498No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGNNNN526210.2041328711375028No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN521140.20216606386157276No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNN503590.19535788482950728No Hit
GAGAGAGACGGGGAGGGCGGCGCCGCCGCNNNNNN494690.19190530400982736No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN466180.18084540747397626No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN455950.17687687918349024No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGNNN448680.17405662496336968No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN446670.17327688480072284No Hit
ACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNN441480.17126352587329152No Hit
GTCTAGGGGTATGATTCTCGCTTNNNNNNNNNNNN431860.16753163514460379No Hit
AGACTGTGAAACTGCGAATGGCTCNNNNNNNNNNN425520.16507215622361832No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGNNNN402970.15632432504566524No Hit
CCAGGTGGCCCGGGTTCGACTCCCGGTATGGGNNN382480.14837563055181785No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGNN370650.1437864135746478No Hit
AAGCCGTGTGTTCGAAAGGGAAACNNNNNNNNNNN345440.13400668745508248No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNNN328800.12755152511356857No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN313440.12159291372140187No Hit
AACTCGGAGGAAGGTGGGGATGACGTCNNNNNNNN310440.12042912243386932No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTNN305060.11834205672489424No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN303010.11754679934508033No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTNNNNN296630.11507180320692775No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN289650.11236404881126867No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNN289470.11229422133401672No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN284160.11023431075508408No Hit
GTCTAGCGGTTAGGATTCCTGGNNNNNNNNNNNNN276660.10732483253625268No Hit
ACTGCGAAGGGGGGACGGAGAAGGCNNNNNNNNNN263170.10209165104664794No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)