FastQCFastQC Report
Tue 19 Sept 2023
SRR15513203_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513203_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193570353
Total Bases6.8 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC63

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN102276615.28369186783474No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN56875092.9382128574203716No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN39934862.063066961499006No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN38263181.9767066292429605No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN29304841.5139115854172152No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNN28851691.490501492240395No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27052461.3975518244779974No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN20946051.0820897764235622No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNN20197231.0434051334297045No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN19748491.0202228643970082No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN18915730.9772018135442466No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNN18864540.9745572970050843No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN18763200.969321991162562No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN17675530.9131320848497911No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN17159510.8864740769471035No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN15733900.8128259186467466No Hit
GACCTGGTATTGCAGTACCTCCAGGNNNNNNNNNN13942670.7202895373135988No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN13823320.7141238203972279No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN12068170.623451360859997No Hit
ATTGATCATCGACACTTCGAACGCANNNNNNNNNN11217500.5795050650137524No Hit
ATTGATCATCGACACTTCGAACGCACNNNNNNNNN10095030.5215173627337447No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN10009390.5170931315086251No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN9144620.47241841833082776No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN8969600.4633767444749145No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGNNNNNNN8608200.44470653003355326No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN7964700.41146280288076964No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNN7773910.4016064381511976No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNN7670200.39624869620401015No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN7047700.36408984592800736No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNNN6996170.3614277647156019No Hit
CGACTGCATAATTTGTGGTAGTGGGGGANNNNNNN6814640.3520497790278866No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNN6745340.3484696853345099No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNNN6652610.3436791790114678No Hit
ATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNN6367150.3289320859997605No Hit
GTCTAGGGGTATGATTCTCGCTTNNNNNNNNNNNN6056120.3128640262385635No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGANNNNNN5783550.29878284098598507No Hit
TTGATCATCGACACTTCGAACGCACNNNNNNNNNN5519090.2851206248510587No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN5508120.2845539058349498No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGC5411150.2795443577044053No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGNNNN4603290.23780966086268387No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN4385920.22658015197192927No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN4174540.21566009129507557No Hit
ACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNN4118680.2127743188028386No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN4078840.21071615238517438No Hit
TTGATCATCGACACTTCGAACGCANNNNNNNNNNN4022740.20781798130005993No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTNN3888840.20090059969049084No Hit
AGTTCAACTTTAAATTTGCCCACAGAACNNNNNNN3768820.1947002700356702No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC3672080.18970260388996657No Hit
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGNN3653890.18876289387145975No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCNNNNNNNNN3602500.18610804517156612No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN3554680.18363762554072524No Hit
GACTGCATAATTTGTGGTAGTGGGGGANNNNNNNN3532310.1824819733629354No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGNNNNNN3507470.18119871900011464No Hit
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGNNNNN3462360.17886830014718214No Hit
AACCTGCATTAAAAATTTCGGTTGGGNNNNNNNNN3459910.1787417311782244No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTNNNNN3378510.17453654176060732No Hit
AGATACCATGATCACGAAGGTGGTNNNNNNNNNNN3326230.17183571494545966No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN3220760.16638704998383713No Hit
ATGCTCATAAGGAAAGGTTAAAAANNNNNNNNNNN3147160.16258481483473866No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN3121460.1612571321807736No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN3062750.15822412639811634No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGNNN3025550.15630234450210462No Hit
CTCTAGCATCACCAGTATTAGAGGNNNNNNNNNNN2976510.15376889869080312No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN2940110.15188844543771637No Hit
ACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNN2937360.15174637822766177No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTNNNNNN2922960.15100246265501205No Hit
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCGNNN2745360.14182750392566573No Hit
GAGAGAGACGGGGAGGGCGGCGCCGCCGCNNNNNN2719120.14047192443772627No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNN2670460.13795810973181416No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGNNNN2657010.13726327192263788No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCNNN2565440.13253269213183694No Hit
ACTGCATAATTTGTGGTAGTGGGGGANNNNNNNNN2525880.1304889907391965No Hit
ATCGTATAGTGGTTAGTACTCTGCGNNNNNNNNNN2410030.12450408663562235No Hit
AAACGATGCCGACCGGCGATGCGGCGGCNNNNNNN2407700.12438371696310334No Hit
AGACCGGGGTTCGATTCCCCGACGGGGANNNNNNN2357100.12176968029809813No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN2301430.11889372335855584No Hit
CTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNN2212810.11431554293854079No Hit
AGTCCGCCCGGAGGATTCAACCCGNNNNNNNNNNN2209470.114142995854329No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN2183120.11278173367798734No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGNNN2123930.10972393070957515No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN2107530.10887669352961298No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAANNNNNNN2089640.10795248175220304No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGNN2056480.10623940950296247No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)