FastQCFastQC Report
Sat 16 Sept 2023
SRR15513204_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513204_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35781045
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN16996114.750031755640451No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN9600642.6831636694791894No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN7888822.2047483520953626No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN7368372.0592942436421295No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNN5165181.4435520259399914No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5018321.4025079479931344No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN4965941.387868912157261No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN3871131.0818940587118124No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN3639481.0171530764403331No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNN3597541.00543178657862No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNN3532470.9872461802051896No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN3325050.9292769397875328No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN3302680.9230250262394516No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN3191090.8918381226708164No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN3105120.8678114347973905No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN2788020.7791890929960262No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN2673280.7471218350386357No Hit
GACCTGGTATTGCAGTACCTCCAGGNNNNNNNNNN2520500.7044232497960862No Hit
ATTGATCATCGACACTTCGAACGCANNNNNNNNNN2063650.576743915668198No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN1940440.5423094825765988No Hit
ATTGATCATCGACACTTCGAACGCACNNNNNNNNN1824910.5100214373280602No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN1723220.48160136183837005No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN1652400.4618087593584816No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGNNNNNNN1609230.44974371206877833No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNN1276560.3567699042887093No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN1272560.35565199395378194No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN1260780.352359748017421No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNN1253260.3502580765877576No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNN1246000.34822906932986447No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN1218230.3404679768296314No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNNN1119470.3128667706602756No Hit
ATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNN1115240.31168458048108993No Hit
CGACTGCATAATTTGTGGTAGTGGGGGANNNNNNN1090860.3048709169897078No Hit
GTCTAGGGGTATGATTCTCGCTTNNNNNNNNNNNN1080090.30186094341291597No Hit
TTGATCATCGACACTTCGAACGCACNNNNNNNNNN1075830.30067036890621834No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNNN1031560.2882978962744101No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGC944500.26396657783471666No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGNNNN937390.2619794922143833No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGANNNNNN934720.26123328706581933No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN869880.24311196053664724No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN820150.2292135402976632No Hit
TTGATCATCGACACTTCGAACGCANNNNNNNNNNN793980.2218996119314011No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN764700.21371650827973301No Hit
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGNN733450.20498283378811324No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN689770.19277525293070674No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCNNNNNNNNN663530.18544176113358343No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTNNNNN658380.1840024515773645No Hit
AACCTGCATTAAAAATTTCGGTTGGGNNNNNNNNN644770.18019876166277424No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN638840.17854145959124448No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTNN633660.1770937657075136No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC630910.17632520235225105No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN608210.1699810612015384No Hit
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGNNNNN604980.1690783486060846No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN595640.16646802797402926No Hit
AGATACCATGATCACGAAGGTGGTNNNNNNNNNNN593480.1658643563931685No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN579590.16198241275513334No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGNNN575470.16083096511015818No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTNNNNNN575360.16080022257594767No Hit
GACTGCATAATTTGTGGTAGTGGGGGANNNNNNNN573320.16023008830513474No Hit
AGTTCAACTTTAAATTTGCCCACAGAACNNNNNNN572650.1600428383240344No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGNNNNNN557030.15567739846614317No Hit
ATGCTCATAAGGAAAGGTTAAAAANNNNNNNNNNN552430.15439180158097673No Hit
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCGNNN549940.15369590239748449No Hit
ACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNN549650.15361485389820223No Hit
GAGAGAGACGGGGAGGGCGGCGCCGCCGCNNNNNN534840.14947579088313379No Hit
CTCTAGCATCACCAGTATTAGAGGNNNNNNNNNNN521200.1456637166410316No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCNNN520330.14542057114318488No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN519890.14529760100634287No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN506850.14165321331447978No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGNNNN484030.13527553485371935No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNN478040.13360146412716567No Hit
AAACGATGCCGACCGGCGATGCGGCGGCNNNNNNN465350.1300548935896087No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN435860.12181309964535691No Hit
ACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNN428270.1196918647848323No Hit
ATCGTATAGTGGTTAGTACTCTGCGNNNNNNNNNN426980.11933133870181824No Hit
AGACCGGGGTTCGATTCCCCGACGGGGANNNNNNN392470.10968656728723267No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGNNN370150.10344862761833816No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGNN363860.10169071361666492No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)