FastQCFastQC Report
Sat 16 Sept 2023
SRR15513206_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513206_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41965339
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN12120612.888243080795797No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN11569852.757001438734952No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN11078492.63991433501824No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN10543682.512473448623875No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN6291981.4993278143183832No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN6090001.4511976181105077No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNN6082761.4494723848173847No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN5553491.3233516355009072No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN5262211.2539419733985706No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4904041.16859296668615No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGNNNNNNN3588910.8552081516605883No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN3528500.8408129385062277No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCNNNNNNNNN3380220.8054790168619869No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN3279510.7814806404876177No Hit
ATTGATCATCGACACTTCGAACGCACNNNNNNNNN3181370.7580946742739287No Hit
ATTGATCATCGACACTTCGAACGCANNNNNNNNNN3056750.7283987387782094No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNN3005100.7160909625917713No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNN2834950.6755455972844637No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN2797290.6665715246575274No Hit
TTGATCATCGACACTTCGAACGCACNNNNNNNNNN2325400.554123964064725No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN2224260.53002312217709No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN2147400.5117080074105919No Hit
TTGATCATCGACACTTCGAACGCANNNNNNNNNNN1644360.39183765440331597No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGC1414700.33711153864383175No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNN1366450.3256139548878659No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN1279970.30500647212691406No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN1264710.30137013786544176No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNN1211250.2886310533557229No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN1209920.2883141251402735No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN1189580.2834672680709192No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNNN1189570.2834648851520061No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN1176160.2802693908894672No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN1106750.26372955071326837No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN1099110.2619090006636191No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN1097130.26143718271881466No Hit
GACCTGGTATTGCAGTACCTCCAGGNNNNNNNNNN1096320.2612441662868492No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN1068500.2546148858704561No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN1017860.24254778449424655No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGANN967220.23048068311803702No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN921940.21969082627927775No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGNNNNNNNNNN874000.20826711300961967No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN852020.20302945723850818No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNNN834870.19894275130244984No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCNNNNNNNNNN826390.19692203606409567No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN824020.19635728428167828No Hit
CTAAACCATTCGTAGACGACCTGCTTNNNNNNNNN776300.18498599522810957No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN773470.1843116291756871No Hit
GTCTAACACGTGCGCGAGTCGGGGNNNNNNNNNNN760410.18119953707510858No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACANNN746680.17792778940734877No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN694540.1655032501941662No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN648060.15442744308582854No Hit
ATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNN645170.15373877951992715No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGNNNN636790.1517418934707045No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC619510.14762420958877515No Hit
AACGATGCCGACCGGCGATGCGGCGGCNNNNNNNN595210.14183371662981203No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNN580880.13841899382726303No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGANNN577070.1375111017213515No Hit
CTCTAGCATCACCAGTATTAGAGGNNNNNNNNNNN548980.13081748249430322No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN533220.12706200228717324No Hit
AGTCTAACACGTGCGCGAGTCGGGGNNNNNNNNNN522120.1244169622935728No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN518960.12366395991701629No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN517340.1232779270530854No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN515630.12287044791893616No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN508370.12114044878798669No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGNNN493690.11764232382347728No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGNNNNNNNN488810.11647945939385834No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN485400.11566688404447299No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNN473750.1128907835106491No Hit
GTCTAGGGGTATGATTCTCGCTTNNNNNNNNNNNN473370.11280023259194927No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNN473260.11277402048390459No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN469670.11191855259408247No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG466360.11112980643382864No Hit
AAACGATGCCGACCGGCGATGCGGCGGCNNNNNNN465840.11100589465034465No Hit
ATGCTCATAAGGAAAGGTTAAAAANNNNNNNNNNN463670.11048880124619033No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNN452360.10779371995541369No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTNN434630.10356880472239245No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN432100.10296592623736461No Hit
CGACTGCATAATTTGTGGTAGTGGGGGANNNNNNN430340.10254653250864959No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTNNNNNN422840.10075934332378442No Hit

[OK]Adapter Content

Adapter graph