FastQCFastQC Report
Sat 16 Sept 2023
SRR15513208_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513208_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182236906
Total Bases6.4 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN60614383.326130877134185No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN52123192.860188484543301No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN46399442.5461055621741076No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN40258202.209113449281234No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN38214592.096973156469195No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37265472.0448914996394856No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN36419831.998488165728626No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN34366781.8858298658779908No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN33599311.843716003387371No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN27882721.5300259761872823No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN27718151.5209954233968392No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN23191081.2725786729500335No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN22261201.221552784703226No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN20610881.1309937406421946No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN20556291.1279981893459057No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN17542680.9626304783730252No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN15762860.8649652996193866No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN14187520.7785206801085616No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN13328130.731362833826865No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN10024030.5500548829554865No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGANNNN9672430.5307613157128557No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNN6693530.3672982683321017No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN6415640.35204943613342515No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGANNN5886200.3229971430704602No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN5841800.32056075403299483No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN5824820.31962899984704524No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN5740890.3150234563354582No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN5724670.31413340610600576No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNN4863510.26687843350457235No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGANNNNN4709390.2584213101159652No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN4552880.2498330387588999No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN4551300.2497463384282874No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN4411050.24205031224575332No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN4231060.23217360812743384No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN3886560.21326964363628959No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN3768530.20679290944502757No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNN3653980.20050713547562093No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN3592520.19713460236204847No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN3456420.1896663017314396No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN3297580.18095017482353437No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCNN3214800.17640773598296275No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCNNN3157650.17327170820163068No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNN3010020.16517071465205846No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN2951170.1619414017048775No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN2924060.16045377767772243No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNN2752950.151064351366896No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN2674320.14674963807824964No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN2666070.14629693065574764No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN2649580.14539206454701334No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNN2617870.14365202183579653No Hit
GTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGNN2592970.14228566852424504No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNN2515950.13805930177502027No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGNNNNN2430660.1333791301307541No Hit
ACGATTAAAGTCCTACGTGATCTGAGTNNNNNNNN2337680.12827698029508908No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGNNNN2327150.12769916100309558No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN2294760.12592180422553925No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN2173040.11924258635075816No Hit
GAGAGAGACGGGGAGGGCGGCGCCGCCGCNNNNNN2163390.11871305585049825No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN2098860.11517206070212802No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCNNNN2098740.11516547586689163No Hit
CGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNN2078100.11403288420623209No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGNNNN1965830.10787222210631693No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTNN1931260.10597524082196612No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGNNN1928880.10584464158977765No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNN1887700.10358494563115553No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTNNNNN1870310.10263069325814828No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN1851880.10161937231309229No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGNN1842350.10109642664806875No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)