FastQCFastQC Report
Sat 16 Sept 2023
SRR15513209_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513209_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142533162
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN54600703.830736597283936No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN47249123.3149562766312584No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN37850022.65552377207488No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN35938052.521381655730054No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN32228352.2611123999339884No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN30816982.1620919347877794No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN29637552.0793441739544094No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28948532.0310031429738435No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN26861731.884595109171857No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN22160611.5547687070886704No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN19764381.3866513394265398No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN17835301.2513088006845732No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN17622741.2363957799518963No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN17069631.197590073810332No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN16314031.1445778491885277No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN15743031.1045169965428818No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN15404111.0807386704856796No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN12150850.8524928395260044No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN10250870.7191919309276251No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN7818990.5485733909418217No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGANNNN7532970.5285064818810377No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN6685720.46906417469360573No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN5254250.36863351140698053No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNN5137360.36043261286801453No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN4644970.3258869679745125No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGANNN4631670.3249538517920482No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN4509490.3163818115534405No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN3905600.2740134257317606No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN3775450.2648822173747889No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNN3705690.25998791775909663No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN3693030.259099703407969No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGANNNNN3566840.2502463251323927No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN3475130.2438120330200771No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN3394370.23814598317828659No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN3173060.22261907022030425No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN3054030.21426803118280643No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN2897750.20330356524329404No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNN2794880.19608629744704603No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNN2713520.19037815213837744No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN2699660.18940574685349365No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN2523110.17701915572461657No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN2485450.17437696358690197No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCNN2416230.1695205498914No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCNNN2360200.1655895348761013No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNN2281100.1600399491593402No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN2239230.15710238716236435No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN2177730.15278760180736045No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN2142450.15031238835492894No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNN2097960.1471910094859188No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN2016260.1414590100793526No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNN1957770.1373554036498538No Hit
GTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGNN1893000.1328111980003643No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN1876930.1316837410791462No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN1864020.1307779869501527No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGNNNNN1792440.12575599775159693No Hit
ACGATTAAAGTCCTACGTGATCTGAGTNNNNNNNN1758050.1233432259083679No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGNNNN1751460.12288087736382358No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN1706150.11970196802341339No Hit
CGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNN1634670.11468699473600397No Hit
GAGAGAGACGGGGAGGGCGGCGCCGCCGCNNNNNN1599600.11222651469697978No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCNNNN1558750.1093605149936967No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN1550820.10880415323979131No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNN1497710.10507800282996597No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTNN1486980.10432519556396287No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGNNN1469510.10309951588669589No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGNNNN1467970.10299147085504215No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGNN1444690.10135816674017237No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGANN1435110.10068604245235226No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTNNNNN1430830.10038576145528855No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)