FastQCFastQC Report
Sat 23 Sept 2023
SRR15513210_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513210_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324436204
Total Bases11.4 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN128063793.947271864887188No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN123828243.816720775095741No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN100073043.0845213563157086No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN90906222.8019752074278372No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN82771882.551252880520079No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN67913182.0932676181848064No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN65323102.0134343576526375No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN52435351.6161990971883027No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN36715441.1316690168153982No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34959591.0775489778569842No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN34083651.0505501414385923No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN31474510.9701294002317942No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN30315920.9344185274711203No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN28903170.8908737571100419No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN28403740.875479975718123No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN26970090.8312910109131963No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN23669270.7295508241120957No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGANNNN19202430.5918707518843982No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN17065240.5259967842553108No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN15574350.4800435280644573No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN14853420.45782251847577404No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN14715620.45357515032446877No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN14448020.44532699562715883No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNN13738580.4234601388690887No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNN11287370.34790722677793384No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN9680330.29837391390512014No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGANNN9222970.2842768435300766No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN9039350.2786171792344112No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN8652460.26669218457506055No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN8607980.2653211908495884No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNN8051830.24817914587608722No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN7155490.22055152636417855No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCNNN7135510.2199356888049399No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNN6934170.2137298462535334No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN6836900.21073172216008298No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCNN6816080.2100899935322878No Hit
GAGAGAGACGGGGAGGGCGGCGCCGCCGCNNNNNN6708820.20678395065921804No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNN6194340.19092628762232713No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN6129490.18892743548435798No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN5811250.17911841922549432No Hit
GTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGNN5622780.1733092648316154No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN5506730.1697322904197215No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNN5248560.16177479378965978No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN5214650.16072959601019127No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN4886060.1506015647994698No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN4656460.1435246727273384No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTNN4580330.1411781405258952No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN4510150.13901500339339443No Hit
AAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACNNN4501700.13875455157279548No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGNNNN4423420.1363417505649277No Hit
ACGATTAAAGTCCTACGTGATCTGAGTNNNNNNNN4280720.13194335118037565No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN4102230.12644180733910942No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGNNN4077260.12567216450356447No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGANNNNN3850360.11867849372322209No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN3733420.11507408710773843No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNN3698640.11400207357869345No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGNNNN3666550.11301297311443084No Hit
AACCTGCATTAAAAATTTCGGTTGGGNNNNNNNNN3666070.1129981782181128No Hit
ACGGAGTCAAGACCCGCAACGCGGNNNNNNNNNNN3466200.1068376450366803No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN3457620.10657318626499526No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGANN3420150.10541825967116789No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNN3387080.10439895296025595No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)