FastQCFastQC Report
Sat 16 Sept 2023
SRR15513211_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513211_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126244399
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN45018493.5659791924709463No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN38151643.0220461503404996No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN31828972.521218386884633No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN31617412.504460415705254No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN31340782.482548156453262No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN30356952.404617570400094No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN29753212.3567944586595084No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN25915682.0528182006712234No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN25891962.0509393054340572No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25318582.005521052858749No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN17599771.3941030366028357No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN16339851.2943029654725513No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN15366151.2171747912554918No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN14355201.1370959910863054No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN13841221.0963828977474082No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN13774041.0910614735470363No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN13156381.042135738631858No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN10268020.8133445983611518No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN9053330.717127260433946No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN7226780.5724436139143091No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGANNNN6575260.5208357798115067No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN5468770.43318911914658487No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN4995830.3957268630982987No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNN4341890.34392733732290176No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN4329110.3429150151841588No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN3956990.3134388560081782No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN3918740.31040901862109543No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGANNN3742980.2964868168131562No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN3458620.2739622531689505No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN3344210.26489967289558725No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN3297480.261198122540074No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGANNNNN3222320.25524459108875No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNN3208660.2541625628872454No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN3080600.2440187465267271No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN2925550.2317370135367352No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNN2796530.22151715419865872No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN2771580.21954082889649623No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN2714310.21500439001654242No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN2358740.18683918008909053No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNN2329780.1845452169327528No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN2310120.18298792012150972No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN2229680.17661615229361582No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNN2195210.17388573413066824No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCNN2069810.163952620187134No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN2064330.16351854152357287No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN2031630.16092832760049813No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCNNN2015620.15966015252684596No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNN1894940.150100916556306No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN1856770.1470774160840197No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN1855570.14698236236207202No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN1734740.13741124467628857No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN1664320.13183317542665793No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNNN1650510.13073926550990989No Hit
GTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGNN1632490.1293118754519953No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNN1574610.1247271175967181No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGNNNN1557990.12341062354774252No Hit
ACGATTAAAGTCCTACGTGATCTGAGTNNNNNNNN1540740.12204422629474437No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGNNNNN1522720.1206168362368298No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN1506490.11933123464748722No Hit
CGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNN1478920.1171473753857389No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN1455410.11528511454991362No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTNN1441780.1142054626914577No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNN1367080.10828836850021362No Hit
GAGAGAGACGGGGAGGGCGGCGCCGCCGCNNNNNN1352460.10713029732115087No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCNNNN1340430.1061773837586252No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGNNN1338160.10599757380127416No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTNNNNN1289270.10212492674625509No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGNN1271140.10068882343049532No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)