FastQCFastQC Report
Sat 16 Sept 2023
SRR15513213_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513213_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52862065
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN927554017.546684943162926No Hit
GTCTAGCGGTTAGGATTCCTGGNNNNNNNNNNNNN12307082.328149685412403No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGNNNNNNN10921902.0661130056118693No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN9063231.7145054776047812No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN8267291.5639362556116567No Hit
GACCTGGTATTGCAGTACCTCCAGGNNNNNNNNNN8029061.5188699117221396No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNN6240661.1805554701656094No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCNNNNNNNNN5670991.072790100046224No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNN5646931.068238631994418No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGANNNNNN4391820.8308074987233284No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN2980370.5638012816941601No Hit
CGACTGCATAATTTGTGGTAGTGGGGGANNNNNNN2698320.5104454394659006No Hit
GTCTAGGGGTATGATTCTCGCTTNNNNNNNNNNNN2600510.49194256788871193No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN2472150.4676605047494834No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGNNNNNNNN2424760.45869566389432576No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTNNNNN2126100.40219768183479027No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTANNNN1988840.3762319916938546No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCNNNNN1733070.32784757840996187No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN1566030.29624835881837003No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCNNN1558480.29482011344051734No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCNNNNNNNN1525360.2885547509352879No Hit
GACTGCATAATTTGTGGTAGTGGGGGANNNNNNNN1459480.2760921276911903No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGANNNNNN1445510.27344940081322966No Hit
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCGNNN1424480.26947112262829687No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTNNNNNNNNN1346580.2547346570740284No Hit
CCGCGGCCCGGGTTCGATTCCCGGTCAGGNNNNNN1327350.25109688772090155No Hit
ATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNN1325670.2507790794778827No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNNN1288240.24369838749205125No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN1249120.23629799554746866No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGANNNNN1237570.23411306387671385No Hit
CCCACATGGTCTAGCGGTTAGGATTCCTGGNNNNN1232030.23306505336104447No Hit
ACCCCTCCCACGGCGCGACCGCNNNNNNNNNNNNN1231350.23293641669125106No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGNNN1222120.2311903630703795No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGNNNN1188200.22477366330656964No Hit
CCCTGGTGGTCTAGTGGTTAGGATTNNNNNNNNNN1164150.22022408697049575No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCNNNNNNNNNN1124470.2127177589449069No Hit
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGNNNNN1123920.21261371457963288No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCNNNNN1123650.212562638254862No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCNNNNNNNNN1109300.2098480261790757No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNNN1082880.20485011321445729No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCNNNNNNN1065880.20163419646962336No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCNNNNNNNN1059210.2003724220762091No Hit
CCCATATGGTCTAGCGGTTAGGATTCCTGGNNNNN1054600.19950034112363943No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGNNNNNN1053550.1993017109717526No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN1007100.19051469139542693No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN982680.18589512157725963No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCNNNNNNNN938470.1775318463249591No Hit
AGATACCATGATCACGAAGGTGGTNNNNNNNNNNN873310.16520542661358387No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGNNNNNN858210.1623489358578784No Hit
CGACCTGGTATTGCAGTACCTCCAGGNNNNNNNNN852440.16125741588036716No Hit
ATGCTCATAAGGAAAGGTTAAAAANNNNNNNNNNN809830.15319681514522748No Hit
GTGGTCTAGTGGTTAGGATTCGGNNNNNNNNNNNN798500.15105350122058228No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGNNN793470.15010196820725788No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCNNNNNNNN749520.14178787756399602No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGN736910.13940242402562217No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTNNNNNN731100.13830333718518184No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN729300.13796282835337592No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTNNNN719790.13616380669200115No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTTNNNNNN714180.13510255416620595No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN691750.13085943577875742No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGNNNNNNN668880.12653308189908966No Hit
GCCGCGGCCCGGGTTCGATTCCCGGTCAGGGNNNN659440.12474730224784067No Hit
TGGGTGGTTCAGTGGTAGAATTCTCGCNNNNNNNN647010.12239589959264739No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGNNNNNNN641750.12140085711748114No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNN633800.1198969431103382No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTANNN625420.1183116853267083No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGNNNN621870.11764012624175767No Hit
GTGGTCTAGTGGTTAGGATTCGNNNNNNNNNNNNN614160.11618161341218887No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGNNNN607900.11499739936379708No Hit
GACCTGGTATTGCAGTACTTCCAGGNNNNNNNNNN594860.11253060204893624No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGNNNNN584140.1105026827839586No Hit
GTGCATTTATCAGATCAAAACCAANNNNNNNNNNN574590.10869609425965482No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC574020.10858826646291628No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGNNNNNN570640.107948866545414No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGNNNNNNN544990.10309661569217925No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN535750.1013486703555754No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)