FastQCFastQC Report
Sat 16 Sept 2023
SRR15513214_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513214_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56672515
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC62

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN48753878.602736264660214No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGNNNNNNN20204323.565100295972395No Hit
GTCTAGCGGTTAGGATTCCTGGNNNNNNNNNNNNN15339172.7066330124929165No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNN14771972.60654922408155No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN12787732.2564253589239867No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGANNNNNN10634751.876526919618796No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNN8096681.4286784343345271No Hit
CGACTGCATAATTTGTGGTAGTGGGGGANNNNNNN5329700.9404382353597683No Hit
GTCTAGGGGTATGATTCTCGCTTNNNNNNNNNNNN4966390.8763313221585455No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN4553590.8034917808041516No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN4114040.7259321383566619No Hit
ATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNN3961420.6990019765313045No Hit
ACCCCTCCCACGGCGCGACCGCNNNNNNNNNNNNN3707380.6541760145989639No Hit
GACCTGGTATTGCAGTACCTCCAGGNNNNNNNNNN3611030.6371748280449526No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGNNNNNNNN3538130.6243114497389078No Hit
GACTGCATAATTTGTGGTAGTGGGGGANNNNNNNN3447040.6082384026895578No Hit
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGNNNNN2962730.5227807518335829No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN2680900.4730511783357418No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN2581310.4554782860792397No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNNN2470700.4359608886247593No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTNNNNN2440130.43056673945033147No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTNNNNNNNNN2423660.4276605687960028No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGNNNNNN2227810.39310237069944753No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN2149220.3792349783664974No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGNNN2116380.3734402822955713No Hit
CCGCGGCCCGGGTTCGATTCCCGGTCAGGNNNNNN1991770.35145255155872296No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGNNNN1856540.32759089657482116No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTANNNN1835470.3238730449848573No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN1832670.3233789783283837No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGANNNNN1802780.318104816770528No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGC1781070.31427403565908446No Hit
CCCACATGGTCTAGCGGTTAGGATTCCTGGNNNNN1693020.29873740383676284No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCNNNNNNNN1686420.29757281814650366No Hit
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCGNNN1674340.2954412734285747No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNN1667260.29419199059720574No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCNNN1560970.27543686741271317No Hit
GTCTAGCGGTTAGGATTCCTGGTNNNNNNNNNNNN1555650.27449814076541335No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGNNNNNN1547820.2731165186510604No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGNNN1541390.27198192986494424No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC1456390.25698347779342423No Hit
CCCTGGTGGTCTAGTGGTTAGGATTNNNNNNNNNN1443540.254716064744965No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCNNNNN1431630.2526145169311791No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCNNNNNNNN1403340.2476226791770226No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN1388350.24497765804111568No Hit
CCCATATGGTCTAGCGGTTAGGATTCCTGGNNNNN1385150.2444130104337173No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCNNNNNNNNN1361990.24032637337517135No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCNNNNNNN1355600.2391988426841477No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGNNNNNNN1314480.23194312092907823No Hit
GTGGTCTAGTGGTTAGGATTCGGNNNNNNNNNNNN1264710.22316108611025995No Hit
CCAGGTGGCCCGGGTTCGACTCCCGGTATGGGNNN1229680.2169799593330206No Hit
CCAGGTGGCCCGGGTTCGACTCCCGGTATGGNNNN1194710.21080941969841993No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGNNNN1181920.20855259379259947No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN1177000.20768444809622444No Hit
ACTGCATAATTTGTGGTAGTGGGGGANNNNNNNNN1169330.2063310583622414No Hit
GCCGCGGCCCGGGTTCGATTCCCGGTCAGGGNNNN1144170.20189151654907145No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGANNNNNN1074860.1896616022775767No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGNNNNNN1042980.18403630048887015No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN1033370.1823405931429018No Hit
ATCGTATAGTGGTTAGTACTCTGCGNNNNNNNNNN1004010.1771599513450215No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNNN984770.1737650076055386No Hit
ACCCCTCCCACGGCGCGACCGCTNNNNNNNNNNNN943270.1664422339470906No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN939440.1657664213419856No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCNNNNNNNN930230.1641412949469421No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGNNNN894370.15781371269653377No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGNNNN857970.15139084616237694No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGN852810.150480351895447No Hit
CGCGGCCCGGGTTCGATTCCCGGTCAGGGNNNNNN805670.14216238682895932No Hit
ATCGTATAGTGGTTAGTACTCTGNNNNNNNNNNNN802650.14162950064947707No Hit
CGACCTGGTATTGCAGTACCTCCAGGNNNNNNNNN802420.14158891660269532No Hit
ATCGTATAGTGGTTAGTACTCTGCGTNNNNNNNNN762380.13452376341512282No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTNNNNNN762210.1344937665109798No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNN760160.1341320391374902No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGNNNN744840.13142878871707037No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGNNNNNNN726580.12820676830735322No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTNNNN723870.1277285823648377No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTNNNN718830.12683926238318521No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGNNNNNNN716280.1263893088210396No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTANNN713240.12585289359401114No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN678310.11968941205450297No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN631990.11151613793741111No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGNNNNN630850.11131498222727544No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNN625700.11040625248411862No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCNNN614440.10841939871558551No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCNNNNNN611440.10789004158364951No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNN601700.10617139542863062No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCNNNNN599850.10584495853060341No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGNNNNNNNN595260.10503504211874133No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN592430.10453568189094838No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCNNNNNNNNN589240.10397279880732309No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGNNNN577920.10197535789615124No Hit
CCACATGGTCTAGCGGTTAGGATTCCTGGNNNNNN573390.10117602862692789No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN570790.10071725244591669No Hit
AGTTCAACTTTAAATTTGCCCACAGAACNNNNNNN566980.10004496888835795No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)