FastQCFastQC Report
Sat 16 Sept 2023
SRR15513215_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513215_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40594219
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC71

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN40067269.870188659129026No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN28620307.050338867216537No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20801135.1241606594278855No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN16973774.181326902729672No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN13370593.2937177581862085No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNN10672902.629167468402336No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN9660612.3797994487835816No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN6561901.6164616937204779No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN5231131.2886391532745094No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN4543271.1191913804278388No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN4035060.993998677496419No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNNN3905620.9621123638319042No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNN3513790.8655887677011349No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNN3046110.7503802450294708No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN2981270.7344075273378212No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN2916250.7183904683570831No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGC2787280.6866199347251883No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN2740480.6750911995621839No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCNNNNNNNNN2517950.620273049224078No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN2217790.546331486264091No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN2132260.5252619837322157No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN1981670.48816556859980476No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN1892520.4662043134762613No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN1807700.44530971269579045No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN1735320.4274795876723235No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN1696320.4178723083698198No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN1604050.395142470902076No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGNNNNNNN1560120.3843207329595379No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1382010.34044502740648863No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNNN1337050.32936955875416646No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGNNN1289730.31771272653379534No Hit
GACCTGGTATTGCAGTACCTCCAGGNNNNNNNNNN1269650.31276620939548067No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG1170110.2882454765295521No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN1165520.2871147736577959No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN1109290.2732630476275452No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGNNNN1002990.24707705301584937No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGANNNN982920.24213299928248405No Hit
TCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNNN942420.23215620923757643No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTNN866820.21353286782041553No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNN843110.20769213468548314No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNN806740.1987327308846612No Hit
GCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNNN802630.19772027145047427No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATN797230.19639003277781994No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG778250.19171449018393483No Hit
ATTGATCATCGACACTTCGAACGCANNNNNNNNNN774130.1906995673447985No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN767140.18897764728519595No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNN760820.18742077535720048No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN715410.17623445348215716No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN681720.16793524220776362No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNN660360.16267340923593088No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN660130.16261675092209557No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNN659550.1624738734350327No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCNN656210.1616510961819465No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCNNN654900.16132839013358036No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGANNNNN652940.1608455627635058No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCNNNNNNNN610510.15039333556337173No Hit
GACCGGCTCCGGGACGGCTGGGNNNNNNNNNNNNN558540.13759102004154827No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTN522220.1286439332654731No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGANNNNNN498160.12271698095731316No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN497370.12252237196631374No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN486270.11978799247252424No Hit
ATTGATCATCGACACTTCGAACGCACNNNNNNNNN485630.11963033455576519No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGNNNNNNNN481950.11872380153440075No Hit
GCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGN479300.11807099922282037No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNN470360.11586871519809261No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG455850.11229431461657138No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTC449400.11070541842423424No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG441200.10868542636575912No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG436210.10745618729602853No Hit
TTGATCATCGACACTTCGAACGCANNNNNNNNNNN434520.1070398718595867No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNNN420790.10365761686411555No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGC409960.10098974930395878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)