FastQCFastQC Report
Sat 16 Sept 2023
SRR15513216_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513216_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217513183
Total Bases7.6 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN141515656.506072323901398No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN124290665.714166759262587No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN103436744.7554239505565965No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN88281594.058677675642308No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN83137083.8221628157590795No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN40305201.8530003305592746No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN38156691.754224248559684No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN32131121.477203338061583No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN28636411.316536754464211No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN28348711.303309969952488No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN24140541.1098426158381398No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN22694711.0433717022108036No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN20031330.920924871022645No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN19967480.9179894167610062No Hit
CGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNN17452100.8023467708621597No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN12088790.5557727505647324No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN11941660.5490085628511078No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN11920830.5480509197458621No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN11808120.5428691648542516No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN11116710.5110821259969333No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNN10611550.4878577865324144No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN9106110.418646349357133No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNN8666180.39842090858465345No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGANNNN7932350.36468364310589857No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN7119480.3273125748888517No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN6819810.313535478904743No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNN6321490.29062560313873026No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN5780270.26574343312331555No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN5665760.26047892462683514No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGANNNNN5341790.24558465497698131No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN5199720.2390530968414912No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN4966730.2283415621755671No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN4906850.22558862558689144No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCNNNNNNNN4801230.220732827950019No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN4572260.21020610966830458No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGANNN4561140.20969487628710762No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN4184830.19239431570453364No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN4135280.190116292859362No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNN4083410.18773160981235792No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCC4070400.18713348514604744No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCNN3962670.1821806818945774No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNNN3887270.17871422533502257No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNN3761890.17294997701357714No Hit
GGGAGAGCGACGCTCAGACAGGCGTAGCCCCNNNN3698790.17004900342063406No Hit
GCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGN3617000.16628877156379068No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN3467580.15941930287508135No Hit
GCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNNN3466450.15936735200091298No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN3420990.15727736373569598No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN3393090.15599468286021081No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG3196990.1469791373518726No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGANN3138270.1442795308641132No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN3123680.14360876692241684No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNN3035880.13957222997375748No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN2994080.13765050737177617No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCNNNN2918800.1341895677192127No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCNNN2915670.13404566839518872No Hit
GACCGGCTCCGGGACGGCTGGGNNNNNNNNNNNNN2888890.1328144786516227No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN2844350.13076678667333924No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCNNN2726980.12537079189356537No Hit
ACCCCTCCCACGGCGCGACCGCNNNNNNNNNNNNN2650030.12183307528537247No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG2646140.12165423555040339No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNN2622870.12058441533633389No Hit
AAGCTTGGGCTGCAGGTCGACCCGNNNNNNNNNNN2607920.11989710067366353No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN2423050.11139784571126432No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN2378260.10933866017674891No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCNNNNNN2377120.10928624956033126No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN2376030.10923613765515996No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNN2331280.10717879108964168No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTN2327610.1070100656841567No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN2320950.10670387734613768No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)