FastQCFastQC Report
Sat 16 Sept 2023
SRR15513217_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513217_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167736144
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN98505515.872646625285484No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN78903804.704042797120697No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN53995983.2191022586044427No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN53293893.177245448065147No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN52648483.1387677541937533No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN43809502.6118103680742775No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN39898482.378645356244746No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN32158971.9172355601545246No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN31857421.8992579202249935No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN27525141.6409784643672265No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN26771251.596033470281754No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22884961.3643427978170286No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN21378721.274544620508267No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN19494691.1622235694174536No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN19015201.1336376016847032No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN18075391.0776085326010594No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN16920621.0087640979752104No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN15926360.9494888591215022No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN14425730.8600251356678379No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN13025750.7765619078497477No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN12411300.7399299700129031No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTN9213600.549291272607292No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG8689580.5180505401388028No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN8337580.49706520021111256No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN8326190.49638615753561144No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGANNNN7559390.4506715022613135No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN7429580.4429325619885479No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN7022920.41868853262776806No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNN6975030.4158334532836286No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN6180460.3684632216178762No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN5830990.34762871382091626No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNN5703000.33999827729436777No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGANN5666190.3378037592184067No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN5624020.33528969164809225No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNN4877140.29076261583788404No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN4808240.2866549740168106No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN4763860.2840091518975183No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN4728310.28188975179970754No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN4591680.27374422056584297No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGANNNNN4510180.26888539896326696No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN4384040.2613652547062248No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN4343490.25894776739353204No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN4098610.24434864795747302No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN3841000.22899059847232447No Hit
GCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGN3836540.2287247046766498No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN3700820.22063342531589378No Hit
GCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNNN3601770.21472831758908204No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNN3553990.21187979616367No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN3549760.21162761437987987No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGANNN3463930.20651064924921608No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTG3435510.20481632151982698No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG3261950.19446911811684428No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNN3221820.19207667013020163No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCNN3107840.1852814739797524No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN3082690.1837820952888961No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN2939300.17523355014051115No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC2919750.17406802913032268No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNN2915420.1738098856022349No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG2593860.15463930063874606No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGANNNNNN2555880.15237503015450266No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN2543690.15164829352462042No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGNNNNNN2501030.14910501340724752No Hit
AGAATCCCGCCCAGGCGGAACGATACNNNNNNNNN2445260.14578014861245409No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGNNNN2440370.14548861931629956No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN2286430.13631111014451364No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG2265270.13504960505113317No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNNN2143200.1277721037870049No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNNN2085540.1243345620249861No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN1998260.11913115160200655No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCNNN1844630.10997212383754332No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGNN1814500.1081758502806646No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN1768200.10541556267085762No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGANN1765640.10526294201683806No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATG1699920.10134488366442954No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)