FastQCFastQC Report
Tue 19 Sept 2023
SRR15513219_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513219_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220943126
Total Bases7.7 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC70

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN147371206.670096629301788No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN128627955.82176745340337No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN104306714.720975569070205No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN88266313.9949787801952255No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN84100953.8064524351846094No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN41209321.8651551078353077No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN39028401.7664455421889884No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN33164121.501025200485305No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN32609531.4759241706392803No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN28838911.3052639619120805No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN28428861.2867048871210414No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN21353750.9664817542230302No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN20230190.9156288483037033No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN20225450.9154143134554908No Hit
CGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNN17939970.8119723082038769No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN17742110.8030170624090834No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN17687830.8005603215734353No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN13296440.6018037420182061No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN11959910.5413117038997628No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN11127490.503635944754398No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNN10959200.49601905243252514No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN9685240.4383589648315196No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNN9035940.4089713114677304No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN8218800.37198713301449354No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGANNNN8143200.36856543796705404No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN7880590.35667957372885184No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN6582720.29793730717831884No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNN6306340.28542820562790444No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN5661370.2562365303005625No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN5650100.2557264442796016No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN5604280.25365260741354767No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN5410460.24488021410541644No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGANNNNN5331810.24132047448265034No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN5303980.2400608743084408No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN5298640.23981918315032802No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCNNNNNNNN4943670.2237530576081376No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGANNN4697870.21262802265230918No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCC4519000.20453227406586075No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN4168730.18867887295122274No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNN4151690.18790763375005384No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNNN3942240.17842781856901943No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCNN3923540.17758144691045966No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN3820230.1729055829507907No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN3806860.1723004498451787No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNN3773800.17080413716967144No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGANN3723730.1685379431084903No Hit
GCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNNN3652150.16529819533738288No Hit
GGGAGAGCGACGCTCAGACAGGCGTAGCCCCNNNN3592240.16258663779383659No Hit
GCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGN3515720.1591233030712166No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN3506460.1587041906884218No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG3388820.15337974352730033No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN3345670.15142675224030278No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN3187280.14425793903178502No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNN3078270.1393240901280631No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNN3040630.13762048428698342No Hit
ACCCCTCCCACGGCGCGACCGCNNNNNNNNNNNNN2947570.13340854062144483No Hit
GACCGGCTCCGGGACGGCTGGGNNNNNNNNNNNNN2898880.1312048060730344No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCNNN2844740.1287544017096961No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCNNNN2836540.1283832654743918No Hit
AAGCTTGGGCTGCAGGTCGACCCGNNNNNNNNNNN2738340.12393868275404052No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCNNN2695640.1220060586994682No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG2694170.12193952574021244No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN2652080.12003451060070545No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN2590860.11726366178054348No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN2521280.11411443504243711No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN2480600.11227323723119587No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCNNNNNN2412940.10921091068477051No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNN2342730.10603316982126884No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTN2304750.104314175404579No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN2246560.1016804659494136No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)