FastQCFastQC Report
Sat 16 Sept 2023
SRR15513221_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513221_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38112173
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC69

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN24316396.380216105757077No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN21336375.598308445965545No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN16846984.420367214433036No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14795203.8820142845174423No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN14072103.692284877065393No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN7088341.8598624644152406No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN6339661.663421290620191No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN5889371.5452726875478866No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN5091091.3358172991080828No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN4956571.3005214895513828No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN4652101.2206336280012162No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN3366740.8833765526830496No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN3268260.8575370394125783No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN3260830.855587531049463No Hit
CGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNN2996310.7861818847222382No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN2678350.7027544716487302No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN2601640.6826270441205229No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN2335470.6127884652496723No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN2078750.5454294091286792No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN1933270.5072578779488642No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNN1849780.4853514912413942No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN1598150.41932796642164694No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNN1493520.39187479548857No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN1349330.3540417388428626No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGANNNN1318000.34582126818116615No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN1315710.3452204102872854No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN1144450.30028463609251566No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNN1048890.27521128223258223No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN974420.255671593430267No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN956920.25107988463423486No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN948080.24876041573383914No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN933950.24505293886024287No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN932080.24456228197746688No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCNNNNNNNN926530.24310605433072527No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGANNNNN887010.23273666395248577No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN832370.2184000371744744No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCC807520.21187981068410872No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGANNN806250.21154658381719668No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNN691740.18150106528955984No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN690330.18113110475227956No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN676720.1775600672257654No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCNN675150.17714812535092134No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN667480.1751356449814604No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNN656900.17235962903505922No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGANN639610.16782302074457942No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNNN639200.16771544356707238No Hit
GCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNNN638390.16750291304565604No Hit
GCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGN606380.15910402169931376No Hit
GGGAGAGCGACGCTCAGACAGGCGTAGCCCCNNNN602990.1582145421096824No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN581500.15257592370815487No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN569230.14935647988373688No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN568660.14920692136866612No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNN534320.14019667679405212No Hit
AAGCTTGGGCTGCAGGTCGACCCGNNNNNNNNNNN518430.1360274052072549No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG515460.13524812662872832No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNN499080.1309502871956422No Hit
ACCCCTCCCACGGCGCGACCGCNNNNNNNNNNNNN494820.12983253408300807No Hit
GACCGGCTCCGGGACGGCTGGGNNNNNNNNNNNNN487430.12789352105428364No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCNNNN485190.12730578232839151No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCNNN483640.12689908812074296No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN472610.12400499966244381No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCNNN465880.12223915965116972No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN461000.12095872885547618No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN460640.12086427084595779No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN448760.11774715653185139No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG433460.1137326911273204No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCNNNNNN430710.11301113688794392No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN410250.10764277334698287No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNN400850.10517636976511416No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGNNNNN396810.10411634099163015No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTN390260.10239772998511525No Hit
GAATACAAGCTTGGGCTGCAGGTCGACNNNNNNNN386800.10148988356029975No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)