FastQCFastQC Report
Sat 16 Sept 2023
SRR15513222_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513222_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45550185
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN26336835.7819369998167955No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN20174144.428991891031837No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN14368663.1544679785603504No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN14039033.0821016423972813No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN13776673.0245036326416677No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN11226712.464690319040417No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN11064282.4290307492713805No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN8119331.7825020908257563No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN7967071.7490752232949218No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN7556321.6588999583646038No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN7362231.6162898130929655No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6498251.4266133057417878No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN6368541.3981370218364646No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN5347571.173995231852516No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN5086271.1166299324580131No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN4854351.0657146617516482No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN4831431.060682849037825No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN4283360.9403606154398715No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN3845300.8441897656398103No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN3475110.7629189650931165No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN3270020.7178939009797656No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTN2432460.5340175896102288No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG2294200.5036642551506652No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN2116240.4645952590532838No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN2056340.45144492826977545No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGANNNN1990830.43706298887699363No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN1952160.42857345145799075No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN1817470.3990038679316012No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNN1813020.39802692349109015No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN1640770.36021148981063417No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN1518490.3333663738138495No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN1511870.3319130317472915No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNN1484810.3259723313966782No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGANN1479220.3247451135489351No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN1310340.2876695231863493No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNN1299180.28521947825239347No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN1287770.28271454879930785No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGANNNNN1251940.27484849951761997No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN1246910.2737442229927277No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN1242420.2727584970291559No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN1169070.2566553791164624No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN1158760.254391941547548No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN1112540.24424489165082425No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN1057150.23208467759241813No Hit
GCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGN1051990.23095186111757837No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN999110.21934268763123574No Hit
GCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNNN988350.21698045792788767No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN969560.2128553374700893No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNN969240.2127850852856031No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGANNN946760.2078498693254484No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTG901090.19782356537081025No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNN874130.1919048188278489No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCNN857060.1881573038616638No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG838840.18415732010748145No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN831310.18250419839129084No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNN792490.17398173026081015No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC776370.1704427764673184No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN754520.16564586949537086No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG700200.15372056117884045No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN693630.1522781960161084No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGANNNNNN682120.14975131275537082No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGNNNN671870.14750104747104759No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGNNNNNN657020.1442409070347354No Hit
AGAATCCCGCCCAGGCGGAACGATACNNNNNNNNN646950.1420301586041857No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN617000.13545499321243154No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG606750.1332047279281083No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNNN573640.12593582221455302No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNNN571780.12552748139222705No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN547190.12012904009061653No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCNNN506880.111279460226122No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGNN480870.10556927485585405No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN473290.10390517623583746No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)