FastQCFastQC Report
Sat 16 Sept 2023
SRR15513223_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513223_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43881354
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN42549669.696523949557253No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN33573487.650967196682218No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN21271474.8474962737020375No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11498912.6204546924418057No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN10447822.380924708932181No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN10310712.34967909148838No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN9550982.1765463299058636No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNN7830961.7845757448596504No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN7302541.6641555773324588No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN6534931.48922706441556No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN5894101.3431900939064003No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN5186591.181957603222544No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN4785861.090636355478001No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN4377160.9974988465488098No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGNNNN3384910.7713777473685065No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN3023900.6891081802079307No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTNN2542620.579430616475508No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN2408530.5488732184517369No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN2279050.5193663805360245No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN2102410.4791123810810396No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN1981810.4516291817248848No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN1719730.3919044977509126No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN1617720.3686577219107688No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN1609410.3667639790695611No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCNNN1604170.3655698500096419No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN1539520.3508369409020515No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCNNN1537980.35048599457528135No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGANN1529230.3484919813549965No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGC1514000.34502125891557495No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN1451740.33083300027615375No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNN1449980.330431918759845No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCNN1425860.32493527888861407No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN1408020.3208697707914847No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN1397330.31843365635435955No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGNNNN1301080.29649951093122606No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGANNNN1210360.2758255818633126No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGNNNN1084460.24713458021372814No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN1084370.24711407036346233No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTNNNNN1034000.23563539083137683No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN987080.22494292222614642No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN977300.2227141851639309No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN969080.220840952172989No Hit
GCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGN962390.21931638663656552No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNNN956350.21793994779650602No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN944990.2153511489185133No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNN940970.21443504227330817No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN911220.2076553973243396No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNN844690.19249406023341942No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN827930.1886746703394795No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNN826430.1883328395017164No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN822740.1874919356408191No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN780700.17791155669444475No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN772870.17612719972132126No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN760600.17333102346841894No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN756060.1722964154661226No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNN739070.16842461151039234No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGN732860.167009431842053No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGANNNNN694980.15837706375240837No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTNNNNN692440.15779823020046282No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNN674850.15378969390962732No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGNNNN671720.15307640689482827No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGANNN659570.15030757710894702No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN634950.14469699362512833No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNN610030.13901804397375706No Hit
GCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGN586310.13361255899259625No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG586200.13358749139782697No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG583770.13303372544065073No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGANN555300.1265457761399067No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG549380.12519668376686827No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN544710.12413245042529909No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNN534730.12185813591804846No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN528140.1203563591041425No Hit
ACGATGCCGACCGGCGATGCGGCGGCGNNNNNNNN526210.11991653675955397No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNN520460.11860618521479532No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATG511980.11667370154530782No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN506050.11532233030001762No Hit
GTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGNN496990.1132576720399284No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN496590.11316651714985823No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN481740.10978239185600336No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGTNNN477720.10886628521079818No Hit
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTCNNN475220.10829656714785967No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGNNNNNNN470790.1072870267403326No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTNNNN465480.10607694557465114No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC459600.10473696869061971No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTN453260.10329216368300759No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC446600.10177443476333936No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNN441370.10058258457567193No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)