FastQCFastQC Report
Sat 16 Sept 2023
SRR15513225_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15513225_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22694124
Total Bases797.1 Mbp
Sequences flagged as poor quality0
Sequence length35-51
%GC63

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1945973785.74790989949645No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN2690291.18545664067051No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN906090.39926194110863233No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN660040.29084180557046396No Hit
GAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNNN497490.2192153352118813No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN496880.21894654316685674No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN487500.21481331467123385No Hit
CTCCATCTAAGGCTAAATACCGNNNNNNNNNNNNN412100.18158885533541633No Hit
ACGATGCCGACCGGCGATGCGGCGGCGNNNNNNNN378880.16695070494899913No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN363570.1602044652615805No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN309920.1365639845803257No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN297800.1312233950955763No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN291750.12855750678016917No Hit
CGCTTCTGGCGCCAAGCGCCCGGNNNNNNNNNNNN251750.11093179890970897No Hit
ATTGATCATCGACACTTCGAACGNNNNNNNNNNNN251550.11084367037035665No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)