FastQCFastQC Report
Wed 28 Jun 2023
SRR1557705.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1557705.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107923199
Total Bases5.2 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1752537316.2387449245273No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1347363012.48446128806838No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA46804004.336787681766179No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA44242684.09945965371171No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATC31856462.9517712869130204No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA29685502.7506134246446865No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA26075032.416072748177155No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT23128392.143041553095549No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG21029441.9485560282548706No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCAT20840541.931052840640871No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATC19183031.7774704769453693No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA17720861.6419880215003635No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAAT17096491.5841348438902372No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA14501821.3437166553967697No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA11832501.096381511078077No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCAT7428280.6882931629926945No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGCTGTAGGCACCA7389150.6846674365165918No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGCTGTAGGCACCAT6779130.6281439081508323No Hit
TCCGCCGCGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAAT6443270.5970236297387738No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA5684190.5266884277587065No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT5061410.4689825771380257No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA4461420.41338841336606413No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATA4142840.3838692735562815No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA4068140.37694768480685975No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCA3221330.29848355403178883No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA3159590.29276281923407405No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA3049730.2825833581897438No Hit
TGGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCGCTGTAGGCACCATCA3029580.2807162897385946No Hit
TGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCA3006940.2786185016624646No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG2790250.258540334780106No Hit
TAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATC2685350.24882045981605866No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCAT2680220.24834512179350798No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGAT2611640.24199060296572567No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAAT2405460.2228862767494503No Hit
TTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAA2404780.22282326898037927No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA2362270.2188843568285999No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1988560.1842569547998665No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGCTGTAGGCACC1962050.18180057839093522No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA1916560.17758554395705042No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGCTGTAGGCACCA1795260.16634606985658384No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1639260.15189134636381563No Hit
TGGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCGCCTGTAGGCACCATC1638800.15184872346120873No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCA1593750.1476744587602523No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCA1499040.13889877374743126No Hit
CGGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCGCTGTAGGCACCATCA1464320.13568167118545105No Hit
TGTCCGGTGCGGAGAGCCATTCGTCCCGGGACTGTAGGCACCATCAATA1426850.13220975779266883No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA1376290.12752494484526908No Hit
TTGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC1246480.11549694704657522No Hit
TCGACAACCCGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATA1165730.1080147744693891No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1164840.10793230841869318No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCTGTAGGCACCATCA1146660.10624777718088212No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCA1141620.105780778421885No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAA1133580.10503580421110385No Hit
GTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1105310.10241634887045926No Hit
TTACCCGTCGCCGCGCTCTCCCCCCGGCCCTGTAGGCACCATCAATAGA1083250.10037230271500756No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)