FastQCFastQC Report
Wed 28 Jun 2023
SRR1557709.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1557709.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124053630
Total Bases6 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1500797412.097972465618298No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT115720939.328298575382277No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA77584266.254090267249738No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA49630684.000743871823823No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATC49027633.9521318320149117No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAAT36026172.9040802756033823No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA30098212.4262256574031733No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT19409961.564642646893928No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCAT15257091.229878561393165No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA14170091.1422551681881457No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA12829951.0342260843153077No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA12079650.9737441782235635No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG11908680.9599622356879037No Hit
TTGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC11155690.8992634878963235No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA10608620.8551640125323217No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG10254570.8266239367602544No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATC9276760.7478023819214319No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAAT7153410.576638507071498No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCAT5883060.47423521584979017No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATA5709380.4602348194083479No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT4749940.38289407573160095No Hit
CCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGAT4705970.3793496409577051No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCA4468360.36019582820752605No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA4208520.3392500485475516No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA4194810.3381448813710651No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA4051240.32657166098243157No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGAT3480650.28057623142506993No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA3249810.2619681503878605No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA3212060.25892511166339915No Hit
TTACCCGTCGCCGCGCTCTCCCCCCGGCCCTGTAGGCACCATCAATAGA3131320.252416636256432No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG2973100.23966247501181545No Hit
TTGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCAT2823120.22757254261725354No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA2782750.22431830491376997No Hit
AGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGG2660900.21449594018329007No Hit
GCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAAT2591310.20888626959162743No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA2525190.2035563167317232No Hit
TGGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCGCTGTAGGCACCATCA2506600.20205777130423352No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGCTGTAGGCACCA2309000.18612917655049674No Hit
TTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAA2239890.18055819890155572No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC2077330.1674541889664978No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA1877090.1513127830277921No Hit
CGGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCGCTGTAGGCACCATCA1734670.13983226448109579No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAG1703940.13735511004393824No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCGCTGTAGGCACCATCAATAGAT1598540.12885878470464748No Hit
CGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATC1580520.12740618714663973No Hit
TAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATC1575260.12698217698264855No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCGCCTGTAGGCACCATCAATAGA1567290.12633971291287485No Hit
TTACCCGTCGCCGCGCTCTCCCCCCGGCCTCTGTAGGCACCATCAATAG1531780.12347724125444778No Hit
TGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCA1521040.12261148666105136No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGTCCGCTGTAGGCACCATCAATAGA1425320.1148954689999801No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCA1416420.11417803735368323No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGCTGTAGGCACCAT1392480.11224822683544207No Hit
TTCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA1385770.11170733174031265No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCAT1359680.10960420908279749No Hit
GGGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC1268540.10225738658352843No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)