FastQCFastQC Report
Thu 29 Jun 2023
SRR1557713.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1557713.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104791296
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1074921210.257733619402893No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT102740429.804289470759098No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA88159728.412885741960858No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA41530523.9631650323324568No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAAT34671163.3085915837895543No Hit
TTGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC32344273.0865416532304364No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATC29822462.845890941171297No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG23461652.238893008823939No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA19603091.8706792212971581No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCAT13829081.3196783061066446No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA12316061.1752941771041747No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT12243991.168416697508923No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG11639411.1107229745493366No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA10813281.0318872284965346No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA10678481.0190235647052213No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA10064710.9604528605123847No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATA9774660.9327740349732864No Hit
GGGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC9356330.8928537347223953No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATC8716460.8317923656560179No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAAT6737430.642937940189231No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCAT6052080.5775365160098793No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA4153720.3963802489855646No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGCTGTAGGCACCA4041000.3856236304206029No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA3434330.3277304634155875No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA3350860.31976510720890405No Hit
GGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG3256930.31080157649734574No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA3236700.3088710726509194No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCA3201950.30555495754151185No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGAT3022480.2884285351332996No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2934160.2800003542278931No Hit
TCGCTTCTGGCGCCAAGCGCCCGTCCGCCTGTAGGCACCATCAATAGAT2659470.2537872992810395No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCGCTGTAGGCACCATCAATAGAT2630360.2510093968109718No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGCTGTAGGCACCAT2528520.24129103241551667No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG2510090.23953229856036898No Hit
TTACCCGTCGCCGCGCTCTCCCCCCGGCCCTGTAGGCACCATCAATAGA2476820.23635741655490167No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC2417710.23071668089685615No Hit
GCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAAT2286290.218175562978055No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA2280470.21762017333958728No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGTCCGCTGTAGGCACCATCAATAGA2270210.2166410843892989No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA2108190.20117987661876038No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCAT2048540.1954876099633313No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCGCCTGTAGGCACCATCAATAGA2002930.1911351492398758No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATA1900170.1813289912933227No Hit
TGGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCGCTGTAGGCACCATCA1857650.1772714023882289No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1775150.1693986111212901No Hit
TTTTGGTGACTCTAGATACCCTCGGGCCGATCGCACCTGTAGGCACCAT1774890.1693737999003276No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1746020.16661880009576369No Hit
CTGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC1715820.16373688135319941No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA1712450.16341528975841657No Hit
TGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG1674680.15981098277475259No Hit
CGGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCGCTGTAGGCACCATCA1628500.15540412822072552No Hit
TGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCA1573200.15012697237755318No Hit
TTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAA1540420.14699884998082285No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGTCCGCCTGTAGGCACCATCAATAG1418970.1354091469581596No Hit
GTGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC1395890.13320667395887537No Hit
TAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATC1364010.13016443655778434No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCA1311100.1251153530919209No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT1249910.11927612766617562No Hit
CTCCCGGGGCTACGCCTGTCTGCGCGTCGCTCTGTAGGCACCATCAATA1242360.11855564798053456No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGA1219630.11638657470177675No Hit
TTGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCAT1170520.11170011677305719No Hit
TTCCCGGGGCTACGCCTGTCTGCGCGTCGCTCTGTAGGCACCATCAATA1118980.10678176935611142No Hit
TTACCCGTCGCCGCGCTCTCCCCCCGGCCTCTGTAGGCACCATCAATAG1088200.10384450250524623No Hit
TCGCTTCTGGCGCCAAGCGCCCGTCCGCTGTAGGCACCATCAATAGATC1083750.1034198489156962No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)