FastQCFastQC Report
Fri 30 Jun 2023
SRR1557715.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1557715.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112903190
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1332027011.797957170209274No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT98871808.757219348718136No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA77854926.8957236726437925No Hit
TTGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC74115366.564505396171712No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA16536301.4646441787871538No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA16226361.437192341509571No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA16189941.433966569057969No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG12736771.1281142720590978No Hit
TTGGGGGGGACGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGAT11145920.9872103702295746No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAAT10917770.9670027923923142No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATC10825410.9588223326550827No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA10593640.9382941261447085No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT9672160.8566773002605153No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA7049000.6243401980050343No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCAT6871490.6086178787330987No Hit
GGGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC6854720.6071325354048898No Hit
TTGTGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC6782270.6007155333697834No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCAT5361050.4748360077337054No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA5354810.4742833218441392No Hit
TTTGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC4943540.4378565388630738No Hit
CCGCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT4610870.4083914723755812No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA4578890.405558957191555No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA4182620.370460746060408No Hit
CTGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC3906950.34604425260260585No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCAT3727940.33018907614567844No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT3555640.3149282141629479No Hit
TCGCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT3257800.28854809151096616No Hit
GTGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC3180160.281671403615788No Hit
CCGCCGCGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA3176260.2813259749348092No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA3069320.2718541433594569No Hit
TGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG2769750.2453207920874512No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2751560.24370967729078338No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATC2701800.23930236160732038No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGTCCGCCTGTAGGCACCATCAATAG2520530.22324701365833863No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG2441050.21620735428290377No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCA2406680.2131631533174572No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGCTGTAGGCACCA2368420.2097744093855984No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG2166600.19189891800222827No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGAT2153260.19071737477036743No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCGCCTGTAGGCACCATCAATAGA2111000.18697434501186372No Hit
GGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG2047270.1813296860788433No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATA1775290.15724002129612102No Hit
ACCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA1685280.14926770448204343No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAAT1578990.13985344435352093No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAAT1564840.13860015824176447No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGTCCGCTGTAGGCACCATCAATAGA1516000.13427432829842983No Hit
TCGCTTCTGGCGCCAAGCGCCCGTCCGCCTGTAGGCACCATCAATAGAT1487310.13173321320681905No Hit
CCTCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA1465330.12978641258940515No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATAGATCG1463050.12958446966821752No Hit
GCCTCGGTTGGCCCCGGATAGCCGGCTCCCCGCTGTAGGCACCATCAAT1461740.1294684410599913No Hit
GGGGGGGGGACGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGAT1293170.11453795061060719No Hit
TCGTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA1291720.11440952199844841No Hit
TTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAA1284900.1138054646640188No Hit
TCCGCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA1252050.11089589231269728No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1247970.11053452076951943No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT1219400.108004034252708No Hit
TTTGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGAT1132560.10031248895624649No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)