FastQCFastQC Report
Wed 28 Jun 2023
SRR1557717.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1557717.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130328182
Total Bases6.3 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1388121210.650967263550104No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT110034468.442875386691115No Hit
TTGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC81640696.264239149748901No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA61514154.719942306875731No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA31510792.417803234606618No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATC17802991.366012302695974No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA15479631.1877423411001007No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAAT14624981.1221655804268027No Hit
GGGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC14459321.1094545921004255No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA14083601.0806258311805501No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA13747641.0548478302260058No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG12412770.9524240889050382No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA12046290.9243043074137258No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT12003960.9210563529536536No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCAT10602480.8135216679382514No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA9230720.7082673799593092No Hit
TTTGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC8595790.6595495976457341No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATC8043240.6171527812764241No Hit
TTGGGGGGGACGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGAT7187680.5515061968715255No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCAT6767950.5193005761409302No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCAT6470680.49649123472005463No Hit
GGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG5687200.4363753036929496No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG5671430.4351652814431188No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA4769800.3659837747142057No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA4658370.357433820414989No Hit
TGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG4634700.35561763609961194No Hit
TTGTGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC4591690.3523175056642776No Hit
CTGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC4522510.3470093674751022No Hit
TCGCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT4492300.34469137304470343No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGCTGTAGGCACCA4430700.3399648435209508No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCGCCTGTAGGCACCATCAATAGA3711100.2847503849934775No Hit
GTGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC3630750.278585179681245No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA3544190.27194348494786796No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGCTGTAGGCACCAT3349030.2569689800476155No Hit
CCTCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA3106870.23838819450424006No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT3053060.23425938681474126No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT2821530.21649423453171474No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATA2716890.2084652726913662No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGTCCGCCTGTAGGCACCATCAATAG2460860.18882025071139255No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCGCTGTAGGCACCATCAATAGAT2453530.18825782438981617No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG2170090.16650965023052344No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAAT2162520.16592880885885447No Hit
TCGCTTCTGGCGCCAAGCGCCCGTCCGCCTGTAGGCACCATCAATAGAT2161270.1658328971396225No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATAGATCG2139070.1641295050060623No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCA2059780.15804563283173856No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGTCCGCTGTAGGCACCATCAATAGA2010510.15426517650649038No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGCTCTGTAGGCACCATCAATAGA1825850.1400963300477866No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAAT1812580.13907813123641977No Hit
GGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG1772610.13601125810225756No Hit
GCCTCGGTTGGCCCCGGATAGCCGGCTCCCTGTAGGCACCATCAATAGA1704070.13075222671332898No Hit
TTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAA1685250.1293081798685721No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCTGTAGGCACCATCAATAGATCGG1674600.12849101202071553No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCA1635570.1254962644994158No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGAT1545530.11858755153969691No Hit
TAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATC1513340.11611763294603465No Hit
TCGACAACCCGCAGTTTTATCCGGTAACTGTAGGCACCATCAATAGATC1507910.11570099243769086No Hit
GCCTCGGTTGGCCCCGGATAGCCGGCTCCCCGCTGTAGGCACCATCAAT1503610.11537105612353282No Hit
GGTGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC1479440.11351650712046302No Hit
CCGCCGCGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA1460740.11208166780075242No Hit
TGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCA1440620.11053787276799425No Hit
ACCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA1342740.10302760150525234No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)