FastQCFastQC Report
Sun 2 Jul 2023
SRR1557719.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1557719.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116631625
Total Bases5.7 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1198100910.272521710985336No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT92129247.8991645705013545No Hit
TTGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC86326737.401657140591156No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA67695175.804186471722399No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA24755752.1225589543144925No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATC15574901.335392523254306No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA15395311.3199944697675265No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA14078571.207097131674192No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAAT13012561.115697393395659No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG12539871.075168934669306No Hit
GGGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC11581540.993001683720003No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA9847910.844360181040091No Hit
TTGGGGGGGACGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGAT9289910.7965172396423353No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT8741600.7495051192161646No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCAT8654710.7420551672841735No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA8415910.7215804461268546No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA7199450.6172811190789805No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCAT5046430.4326811017166228No Hit
GGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG4965690.42575845102046717No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCAT4962510.42548579769852307No Hit
TTGTGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC4766480.4086781779813151No Hit
TTTGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC4705560.4034548948452017No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA4588780.39344217316701197No Hit
CTGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC4505390.38629231136923625No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG4385520.37601465297255354No Hit
TGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG4037200.34614968281544567No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATC3928600.3368383146509362No Hit
GTGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC3725050.31938592984535713No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA3670740.31472938836271896No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA2854670.2447595152686932No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGCTGTAGGCACCA2738650.23481195601964736No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGCTCTGTAGGCACCATCAATAGA2707410.2321334372216798No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2551140.21873484142915783No Hit
TCGCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2516530.2157673787019601No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATA2416440.20718565826378568No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG2402950.2060290251464815No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT2373430.2034979792144712No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGTCCGCCTGTAGGCACCATCAATAG2320990.19900177160354235No Hit
TTTTGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC2309740.198037196172136No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGAT2166090.1857206396635561No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCA2023310.17347867698833827No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAAT1916260.1643002058832671No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCGCCTGTAGGCACCATCAATAGA1818850.1559482687478632No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATAGATCG1783310.15290106778500256No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGTCCGCTGTAGGCACCATCAATAGA1779620.15258468704350128No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAAT1731640.1484708800036011No Hit
CCTCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA1610760.13810662416818767No Hit
TTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAA1603350.13747129048403467No Hit
TCGACAACCCGCAGTTTTATCCGGTAACTGTAGGCACCATCAATAGATC1568250.1344618151380468No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCA1542350.1322411481448535No Hit
GGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG1495230.12820107753793192No Hit
ACCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA1489210.1276849225070816No Hit
TGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG1460600.12523190000996728No Hit
TTTGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGAT1437360.1232393015187776No Hit
TCGCTTCTGGCGCCAAGCGCCCGTCCGCCTGTAGGCACCATCAATAGAT1405040.12046818347939506No Hit
TCCGCCGCGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAAT1401280.12014580093520948No Hit
CCGCCGCGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA1326160.11370500925456539No Hit
GCCTCGGTTGGCCCCGGATAGCCGGCTCCCTGTAGGCACCATCAATAGA1313870.11265126418327791No Hit
TAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATC1248300.10702928986884988No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCTGTAGGCACCATCAATAGATCGG1242010.1064899850276458No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGCTGTAGGCACCAT1233440.10575519289901002No Hit
GGGGGGGGGACGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGAT1228470.10532906490842428No Hit
TGCCGCCTGGATACCGCAGCTAGGAATACTGGAATCTGTAGGCACCATC1189980.10202893083243932No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)