FastQCFastQC Report
Sun 10 Sept 2023
SRR15618737_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15618737_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184717741
Total Bases9.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCCCCGCGTCCGGCGCTCCGGCGGCGGGCGCGGGGGGCTGTAGGCAC85663104.637513404843988No Hit
TTCCTCCCCGCGTCCGGCGCTCCGGCGGCGGGCGCGGGGGGCTGTAGGCA33275021.8013981667305037No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATAGA28572261.5468064867683717No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGCTGTAGGCACCATCAATAGA17982050.9734879769886314No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCAATA15982110.8652179218670718No Hit
TTTCCTCCCCGCGTCCGGCGCTCCGGCGGCGGGCGCGGGGGGCTGTAGGC15154010.8203873606271528No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCAAT13854930.7500595191882516No Hit
TCGTTTCCCGGTCAGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA13579750.7351621953843621No Hit
GTGTAATGGTTAGCACTCTGGCTGTAGGCACCATCAATAGATCGGAAGAG13258640.7177783751697137No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATAG13185970.7138442646935576No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCCTGTAGG12161570.6583866787327157No Hit
TTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCCTGTAGGCACC11901650.6443154802331629No Hit
GTGTAATGGTTAGCACTCTGGACTGTAGGCACCATCAATAGATCGGAAGA10975710.5941881889947972No Hit
AGCAGTTGACCCGGGTTCGTTTCCCGGCCAACGCCTGTAGGCACCATCAA9802330.5306653246695996No Hit
TCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCCTGTAGGCACCA9445410.5113428709589947No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA9421650.5100565841155452Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCCTGTAG9244490.5004657349074012No Hit
GCGCAGCGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATAGATCGGAAG8553960.4630827528363937No Hit
ATCCCAGCGGTGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT8393900.4544176403716414No Hit
TGCCCGGATAGCTCAGTCGGTAGAGCATCAGCTGTAGGCACCATCAATAG8198390.4438333836055303No Hit
AACCATCCTCTGCTACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC7741670.41910809205922456No Hit
GCCCACCCAGGGACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC7320320.39629761388214463No Hit
GCGCAATGGATAGCGCATTGGCTGTAGGCACCATCAATAGATCGGAAGAG6899560.3735190763295443No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG6524430.35321079419220486No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGCTGTAGGCACCATCAATAGA6507530.3522958847791453No Hit
GCCCCACGTTGGGCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC6303570.3412541733065044No Hit
ATCCCGGACGAGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG6091980.32979939918169526No Hit
ATCCCACTTCTGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT6087520.3295579497152902No Hit
TCGGGCCCCACGTTGGGCGCCACTGTAGGCACCATCAATAGATCGGAAGA5605240.3034489253525464No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA5539160.2998715754108318No Hit
ATCTCGCTGGGGCCTCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC5478700.2965984734514483No Hit
ATCTCAGGGTCGTGGGTTCGTGCCCCACGTTGGGCGCCACTGTAGGCACC5393640.29199360986122064No Hit
GCCTAATGGATAAGGCATTGGCCTGTAGGCACCATCAATAGATCGGAAGA5005980.27100699547857726No Hit
ATCTCGGTGGAACCTCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC4973460.26924647156658327No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTAGGCACCATCAATAGA4890000.2647282266190122No Hit
TCGACTCCCGGTGTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA4623060.25027698882480376No Hit
ACCCCGTCCGTGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4599190.2489847469496717No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATA4550850.2463677812084114No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCACTGTAG4544010.2459974865110547No Hit
TCCCCGTACGGGCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC4508490.24407455264407985No Hit
ATCTCAGGGTCGTGGGTTCGGGCCCCACGTTGGGCGCCACTGTAGGCACC4273580.23135731180255176No Hit
TCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGTCTGTAGGCACCATCAATA4153090.22483438664399866No Hit
ATCCCGGACGAGCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC4046220.2190488026810592No Hit
CTCCTGGCTGGCTCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC3923790.21242085241828507No Hit
TCGACTCCCGGTATGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA3908850.2116120508424797No Hit
GCCTAATGGATAAGGCATTGGCTCTGTAGGCACCATCAATAGATCGGAAG3751280.20308173864036155No Hit
ATCCCACCAGAGTCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC3481130.1884567221943235No Hit
GCCTAATGGATAAGGCATCAGCCTGTAGGCACCATCAATAGATCGGAAGA3475940.1881757529722064No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGA3424560.18539421180989865No Hit
ATCTCGGTGGAACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3323390.1799172067614231No Hit
ATCCCTTCGTGGTTGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC3283950.1777820572199397No Hit
GTGTAATGGTTAGCACTCTGGACCTGTAGGCACCATCAATAGATCGGAAG3274120.17724989393411866No Hit
ATCCGAGTCACGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3267200.17687526830462919No Hit
ATCTGAGGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCACTGTAGGCACC3257470.17634851868397416No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCACCTGTA3238970.17534699062825806No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCACTGTA3230300.174877625858363No Hit
ATCTCGGTGGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3188290.17260334512211253No Hit
GCCTAATGGATAAGGCACTGGCCTGTAGGCACCATCAATAGATCGGAAGA3152070.1706425155989754No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAG3096730.16764659329609277No Hit
GTTGGGAGAGCTTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC3028530.16395447365285828No Hit
ACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGCTGTAGGCACCATCAA3008070.16284683775988792No Hit
TCGGGCCCCACGTTGGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCA2991940.16197361356860682No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGGGCTGTAGGCACCATCAATAG2927560.15848829593471478No Hit
TATCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGGCTGTAGGCACCATC2856090.15461914944055105No Hit
GTATAGTGGTGAGTATCCCCGCCTGTAGGCACCATCAATAGATCGGAAGA2836520.15355969516755838No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCCTGTAGGCACCATCAATAG2801500.15166382962641364No Hit
TGCACGAGGCCCCGGGTTCAATCCCCGGCACCTCCCTGTAGGCACCATCA2760020.14941824131554315No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGTAGGCACCATCAATAG2757420.1492774860212263No Hit
ATCCCAGCGGTGCCTCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC2651940.14356715200409473No Hit
ATCCCAGTGGGGCCTCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC2634850.14264195662721968No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGCTGTAGGCACCATCAATAGAT2610150.14130478133120955No Hit
ACCTCAGAGGGGGCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC2555960.13837111617773626No Hit
ACCGAAAGGTTGGTGGTTCGGGCCCACCCAGGGACGCCACTGTAGGCACC2551650.13813778720908026No Hit
ACCGAAAGGTTGGTGGTTCGTGCCCACCCAGGGACGCCACTGTAGGCACC2550810.13809231242168557No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGCTGTAGGCACCATCAATAGATC2539350.13747190639365822No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGCTGTAGGCACCATCAA2488090.13469686162954972No Hit
ATCCCGGACGAGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2482420.13438990681463564No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCACCTGT2445770.13240579853128454No Hit
AACCATCCTCTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2353710.12742197837943461No Hit
ACGCCAAGGTCGCGGGTTCGTTCCCCGTACGGGCCCTGTAGGCACCATCA2331070.12619632458584473No Hit
ACCCAGAGGTCGATGGATCGTAACCATCCTCTGCCTGTAGGCACCATCAA2327640.1260106358706498No Hit
GTCAGGATGGCCGAGCGGTCTAAGGCTGCGCTGTAGGCACCATCAATAGA2257700.12222431845352634No Hit
ATCCCTTCGTGGTTACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC2180360.11803738981411645No Hit
TTCCCCGCGTCCGGCGCTCCGGCGGCGGGCGCGGGGGGCTGTAGGCACCA2162580.11707484014759578No Hit
CCTCCCCGCGTCCGGCGCTCCGGCGGCGGGCGCGGGGGGCTGTAGGCACC2150650.11642898989328807No Hit
GCGCAGCGGAAGCGTGCTGGGCCCCTGTAGGCACCATCAATAGATCGGAA2147030.11623301521427765No Hit
TCCTCACACGGGGCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC2135320.11559907502333519No Hit
TCCCGGGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2132520.11544749239868626No Hit
ATCTCAGGGTCGTGGGTTCGTGCCCCACGTTGGGCGCTGTAGGCACCATC2131340.11538361114972709No Hit
GTCCCGGCGGAGTCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC2124690.1150236024161859No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATA2117360.11462678075951568No Hit
CAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCAAT2057910.11140835681830907No Hit
TTCCCCGCGTCCGGCGCTCCGGCGGCGGGCGCGGGGGCTGTAGGCACCAT2033520.11008796388431363No Hit
ACCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGCTGTAGGCACCATCAA2002150.10838969712172908No Hit
TCCCCGGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1997550.10814066852409157No Hit
GTCCCATCTGGGGTGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1988100.10762907716590146No Hit
GCGCCGCTGGTGTAGTGGTATCATGCACTGTAGGCACCATCAATAGATCG1904640.10311083221833034No Hit
ATCTGAGGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCTGTAGGCACCATC1870190.101245824568632No Hit
GTATAGTGGTGAGTATCCCCGCCCTGTAGGCACCATCAATAGATCGGAAG1867160.10108179051410118No Hit
AGCAGTTGACCCGGGTTCGATTCCCGGCCAACGCCTGTAGGCACCATCAA1852330.10027894396997851No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)