FastQCFastQC Report
Sat 16 Sept 2023
SRR15618738_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15618738_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163480151
Total Bases8.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCAATA57769453.533728691013994No Hit
TCCTCCCCGCGTCCGGCGCTCCGGCGGCGGGCGCGGGGGGCTGTAGGCAC52329113.2009457833202024No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCAAT47917972.93111853071386No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA41130522.5159335704308226Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATAGA25390541.5531267768403274No Hit
TTCCTCCCCGCGTCCGGCGCTCCGGCGGCGGGCGCGGGGGGCTGTAGGCA19016661.1632396889577132No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGCTGTAGGCACCATCAATAGA17457281.067853185430444No Hit
ATCTCGGTGGAACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT15620400.9554921441196857No Hit
ATCTCGGTGGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT15239620.9322000198054625No Hit
ATCCCACTTCTGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT14981010.9163809739813612No Hit
AACCATCCTCTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC14733720.9012543669598152No Hit
GTGTAATGGTTAGCACTCTGGACTGTAGGCACCATCAATAGATCGGAAGA14656870.8965534904601354No Hit
TCCCCGTACGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT14464710.8847991582782426No Hit
TCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGTCTGTAGGCACCATCAATA11873190.7262771613172782No Hit
TCGTTTCCCGGTCAGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA10043240.6143400246798156No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATAG9447540.5779013502379259No Hit
GCGCAGCGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATAGATCGGAAG8610780.5267171547939175No Hit
TCCCCGTACGGGCCACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC8413070.5146233318563548No Hit
TTTCCTCCCCGCGTCCGGCGCTCCGGCGGCGGGCGCGGGGGGCTGTAGGC8152710.49869723939758287No Hit
TCCCCGTACGGGCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC7889870.48261944656510625No Hit
TCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCCTGTAGGCACCA7165250.4382947994707932No Hit
ATCCCAGCGGTGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT6964560.4260186914067629No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA6287160.3845824683633917No Hit
ACCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCA6175350.3777431059505199No Hit
TTCCCGGCCAACGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT6063400.37089517980687453No Hit
TGCCCGGATAGCTCAGTCGGTAGAGCATCAGCTGTAGGCACCATCAATAG6062390.37083339860629316No Hit
GTGTAATGGTTAGCACTCTGGCTGTAGGCACCATCAATAGATCGGAAGAG5860380.3584765467949684No Hit
TCGGGCCCCACGTTGGGCGCCACTGTAGGCACCATCAATAGATCGGAAGA5741990.35123468903573496No Hit
CCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCAA5564810.34039667604662294No Hit
CAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCAAT5420850.3315907140310875No Hit
TTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCCTGTAGGCACC4979660.30460333988803323No Hit
AGCAGTTGACCCGGGTTCGTTTCCCGGCCAACGCCTGTAGGCACCATCAA4715190.2884258407615491No Hit
TCCCCGGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4650170.28444859951224294No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGCTGTAGGCACCATCA4585130.28047013487282624No Hit
TCCCCGTACGGGCCACTGTAGGCACCATCAATAGATCGGAAGAGCACACG4354010.26633263875563706No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGCTGTAGGCACCATCAATAGAT4348680.26600660529118303No Hit
GCGCAATGGATAGCGCATTGGCTGTAGGCACCATCAATAGATCGGAAGAG4285980.26217127729469736No Hit
ATCCGGGTGCCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4149490.2538222514854418No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGCTGTAGGCACCATCAATAGA4099810.2507833504509058No Hit
TTCCCGGTCAGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC3861520.23620726897909458No Hit
GTGTAATGGTTAGCACTCTGGACCTGTAGGCACCATCAATAGATCGGAAG3819970.2336656760244857No Hit
CCGCCGGGTCCGCCCCCCGGGCCGCGGTCTGTAGGCACCATCAATAGATC3752710.22955141508280108No Hit
TCCCGGGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3735300.2284864539915919No Hit
TCGTGCCCCACGTTGGGCGCCACTGTAGGCACCATCAATAGATCGGAAGA3584180.2192425183164897No Hit
TTCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGTCTGTAGGCACCATCAAT3574640.21865896123377085No Hit
GCCCCACGTTGGGCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC3523060.21550383813873525No Hit
TCCCCGGCATCTCCACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC3509870.21469701236084618No Hit
AGCGCTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT3421050.20926393688001915Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCGAGTCACGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3397520.20782461841499034No Hit
ACCCCGTCCGTGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3376390.20653210676322412No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGTAGGCACCATCAATAG3357150.2053552054769022No Hit
TCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCACTGTAGGCACC3293870.20148439916721142No Hit
ATCTCGGTGGAACCTCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC3190120.19513806296887992No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATA3174790.194200334449165No Hit
ACCCCACTCCTGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC3161220.19337026425917603No Hit
AACCGGGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA3032100.1854720577056477Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGTCTGTAGGCACCATCAA3032070.1854702226204819No Hit
AAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCAAT2954040.18069716610428138No Hit
TTAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCAA2951650.180550970986074No Hit
TCGACTCCCGGTGTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA2776860.1698591531151693No Hit
TCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCAA2751650.16831706988085668No Hit
ACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGCTGTAGGCACCATCAA2733750.16722213573193973No Hit
AGCCCGGATAGCTCAGTCGGTAGAGCATCAGCTGTAGGCACCATCAATAG2720360.1664030760529454No Hit
TCCTCCCCGCGTCCGGCGCTCCGGCGGCGGGCGCGGGGGGTGCTGTAGGC2578150.15770416067208062No Hit
ATCCCAGCGGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2557330.15643061156702748No Hit
ATCTCGCTGGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2533970.1550016919179381No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAG2400230.14682088224887924No Hit
TGCCCGCATCCTCCACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2392230.14633152620467058No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG2367800.14483715518466828No Hit
GTATAGTGGTGAGTATCCCCGCCCTGTAGGCACCATCAATAGATCGGAAG2366960.14478577280002636No Hit
AGCGCTTTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG2338980.14307425003540644Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCGGGCGGAAACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2316670.14170955836711943No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCCTGTAGGCACCATCAA2291000.14013933716026478No Hit
ACCCAGAGGTCGATGGATCGTAACCATCCTCTGCCTGTAGGCACCATCAA2284480.13974051198423473No Hit
GCGCAGCGGAAGCGTGCTGGGCCCCTGTAGGCACCATCAATAGATCGGAA2277500.1393135488356626No Hit
TCCCCGTACGGGCCACCCTGTAGGCACCATCAATAGATCGGAAGAGCACA2276820.1392719535719049No Hit
TTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCACTGTAGGCAC2238280.13691448082892949No Hit
TTCCCGGGCGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2221950.1359155828036885No Hit
GCCTAATGGATAAGGCATTGGCTCTGTAGGCACCATCAATAGATCGGAAG2216460.13557976221835027No Hit
GTTGGGAGAGCTTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2161720.1322313434858523No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCCTGTAGG2149460.1314814053481025No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGCTGTAGGCACCATCAATAGA2147930.13138781600464755No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG2128960.13022743048481772No Hit
ATAGCTCAGTCGGTAGAGCATCAGCTGTAGGCACCATCAATAGATCGGAA2105220.12877526642362838No Hit
TGCCCGCATCCTCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC2102880.12863212978069735No Hit
TCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGCTGTAGGCACCATCAATAG2098180.12834463310472474No Hit
TGCCCGCATCCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2075520.1269585321095036No Hit
GCCCACCCAGGGACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2071110.12668877459013356No Hit
CTCAGTCGGTAGAGCATCAGCTGTAGGCACCATCAATAGATCGGAAGAGC2060830.1260599520733254No Hit
CCGGGCCGCGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2010890.12300514696735262Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCCGGCATCTCCACTGTAGGCACCATCAATAGATCGGAAGAGCACACG1983780.12134684167254042No Hit
ATCCGGGTGCCCCCTCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1962240.1200292505235085No Hit
TAAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCAA1956420.11967324400134668No Hit
TCGACTCCCGGTATGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA1956060.11965122297935729No Hit
GCCTAATGGATAAGGCATTGGCCTGTAGGCACCATCAATAGATCGGAAGA1881400.11508430769677966No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCCTGTAGGCACCATCAATAG1867690.114245673776017No Hit
CCCGCCGGGTCCGCCCCCCGGGCCGCGGTCTGTAGGCACCATCAATAGAT1846380.1129421516132561No Hit
CTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATAGATCGGAAGAGC1806250.11048741935649423No Hit
ATCTCGGTGGAACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1780200.10889395373753967No Hit
TCCCCGGCATCTCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1756530.1074460715417372No Hit
AGAGCACTGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1747500.10689371090683664Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCTCCCCGCGTCCGGCGCTCCGGCGGCGGGCGCGGGGGGTGCTGTAGG1731740.10592967949974552No Hit
ATCCCAGTGGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1727670.10568071961225434No Hit
ACCCCACTTCTGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1690520.1034082724819602No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)