FastQCFastQC Report
Sat 16 Sept 2023
SRR15618741_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15618741_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159641048
Total Bases8.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCCCCGCGTCCGGCGCTCCGGCGGCGGGCGCGGGGGGCTGTAGGCAC80358075.033672166822659No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATAGA28456431.7825258826915245No Hit
TTTCCTCCCCGCGTCCGGCGCTCCGGCGGCGGGCGCGGGGGGCTGTAGGC21965931.3759575168912697No Hit
TTCCTCCCCGCGTCCGGCGCTCCGGCGGCGGGCGCGGGGGGCTGTAGGCA20847941.305926029751446No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGCTGTAGGCACCATCAATAGA19009791.1907833378793653No Hit
AGCAGTTGACCCGGGTTCGTTTCCCGGCCAACGCCTGTAGGCACCATCAA14617040.9156191457725835No Hit
TTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCCTGTAGGCACC13539300.8481089400014463No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATAG13477890.8442621849989359No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCAATA12606270.7896634454567099No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCAAT11399520.7140719847942867No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCCTGTAGG10538950.6601654231184951No Hit
TCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCCTGTAGGCACCA9919690.6213746479539523No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCCTGTAG9794320.6135214045951389No Hit
TGCCCGGATAGCTCAGTCGGTAGAGCATCAGCTGTAGGCACCATCAATAG8769810.5493455542837579No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGCTGTAGGCACCATCAATAGA8592710.538251916261537No Hit
GTGTAATGGTTAGCACTCTGGACTGTAGGCACCATCAATAGATCGGAAGA7784440.48762145435176546No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTAGGCACCATCAATAGA7061750.44235176907633433No Hit
GCCTAATGGATAAGGCATTGGCCTGTAGGCACCATCAATAGATCGGAAGA5600080.3507919842771265No Hit
GTGTAATGGTTAGCACTCTGGCTGTAGGCACCATCAATAGATCGGAAGAG5595290.3504919361341201No Hit
TATCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGGCTGTAGGCACCATC4867030.30487334310158126No Hit
TCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGTCTGTAGGCACCATCAATA4805170.3009983998601663No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCCTGTAGGCACCATCAATAG4277370.2679367276516501No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGA4247920.2660919640166732No Hit
GCGCAGCGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATAGATCGGAAG4196410.26286535027006336No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGCTGTAGGCACCATCAA4087620.2560506869135562No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCACTGTAG4069440.25491188206181153No Hit
ACCCAGAGGTCGATGGATCGTAACCATCCTCTGCCTGTAGGCACCATCAA3944440.24708181569943086No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGGGCTGTAGGCACCATCAATAG3912820.2451011221124031No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCACTGTA3785840.23714702749884228No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGCTGTAGGCACCATCAATAGAT3752870.23508176919510076No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCACCTGTA3743250.2344791672878519No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3658340.22916035980921398Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGCTGTAGGCACCATCAA3648070.22851704155688077No Hit
GCCTAATGGATAAGGCATCAGCCTGTAGGCACCATCAATAGATCGGAAGA3641390.22809860281047517No Hit
ACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGCTGTAGGCACCATCAA3561470.22309237158102346No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA3548350.22227052781562798No Hit
ACGCCAAGGTCGCGGGTTCGTTCCCCGTACGGGCCCTGTAGGCACCATCA3515990.22024348023573487No Hit
ATCTCAGGGTCGTGGGTTCGTGCCCCACGTTGGGCGCCACTGTAGGCACC3513460.2200849996925603No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCACCTGT3510890.21992401352814972No Hit
GTATAGTGGTGAGTATCCCCGCCTGTAGGCACCATCAATAGATCGGAAGA3446710.21590374425504902No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAG3388220.21223989960276382No Hit
GTATAGTGGTGAGTATCCCCGCCCTGTAGGCACCATCAATAGATCGGAAG3336190.20898071278008648No Hit
TGCACGAGGCCCCGGGTTCAATCCCCGGCACCTCCCTGTAGGCACCATCA3177920.1990665959546946No Hit
ACCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCA3131330.19614817362010803No Hit
ACCGAAAGGTTGGTGGTTCGTGCCCACCCAGGGACGCTGTAGGCACCATC3106110.1945683794308341No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATA3101910.1943052892010581No Hit
ATCTGAGGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCACTGTAGGCACC3084920.19324102658108333No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGCTGTAGGCACCATCAATAGATC3023720.18940742609006175No Hit
ACCGAAAGGTTGGTGGTTCGGGCCCACCCAGGGACGCTGTAGGCACCATC3022850.18935292882817956No Hit
ATCTCAGGGTCGTGGGTTCGTGCCCCACGTTGGGCGCTGTAGGCACCATC2917680.18276502419352697No Hit
TTGGGGTCGGCGGGGGACCGCCCCCCGGCCGGCGCTGTAGGCACCATCAA2827020.1770860336622195No Hit
TTCCCCGCGTCCGGCGCTCCGGCGGCGGGCGCGGGGGCTGTAGGCACCAT2777830.17400474594729548No Hit
ATCTCAGGGTCGTGGGTTCGGGCCCCACGTTGGGCGCCACTGTAGGCACC2775630.17386693677931755No Hit
TTGCGAGAGGTAGCGGGATCGTTGCCCGCATCCTCCCTGTAGGCACCATC2769010.17345225646476586No Hit
GTCAGGATGGCCGAGCGGTCTAAGGCTGCGCTGTAGGCACCATCAATAGA2644010.1656221901023852No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGTAGGCACCATCAATAG2585460.16195458701824608No Hit
GCGCCGCTGGTGTAGTGGTATCATGCACTGTAGGCACCATCAATAGATCG2538370.15900484441821003No Hit
TTCCCCGCGTCCGGCGCTCCGGCGGCGGGCGCGGGGGGCTGTAGGCACCA2522940.15803830102643776No Hit
CCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCAA2391350.14979543356543237No Hit
ACGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCCTGTAGGCACCATCA2382980.14927113232180736No Hit
ATCTAAAGGTCCCTGGTTCGTTCCCGGGTTTCGGCCTGTAGGCACCATCA2362070.14796131882070832No Hit
ATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAGCGCTGTAGGCAC2303020.1442623954711197No Hit
TTCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGTCTGTAGGCACCATCAAT2223590.1392868581018085No Hit
ATCTGAGGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCTGTAGGCACCATC2219750.13904631846315618No Hit
GCCTAATGGATAAGGCATTGGCTCTGTAGGCACCATCAATAGATCGGAAG2202150.13794384511933297No Hit
ACCGAAAGGTTGGTGGTTCGTGCCCACCCAGGGACGCCACTGTAGGCACC2151660.13478112471424017No Hit
ACCGAAAGGTTGGTGGTTCGGGCCCACCCAGGGACGCCACTGTAGGCACC2074350.12993838527043494No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCCTGTAGGCACCATCAA2071650.12976925583700752No Hit
ATCAGAAGATTCCAGGTTCGGCTCCTGGCTGGCTCGCTGTAGGCACCATC2020050.12653700444261679No Hit
CCTCCCCGCGTCCGGCGCTCCGGCGGCGGGCGCGGGGGGCTGTAGGCACC2016890.1263390603649758No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGA1991830.12476928866064572No Hit
GTCAGGATGGCCGAGCGGTCTAAGGCTCTGCGCTGTAGGCACCATCAATA1949330.1221070660974363No Hit
ACCCAGAGGTCGATGGATCGAAACCATCCTCTGCCTGTAGGCACCATCAA1938280.12141488823100184No Hit
TCCCCGCGTCCGGCGCTCCGGCGGCGGGCGCGGGGGGCTGTAGGCACCAT1883890.11800786975540276No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGCTGTAGGCACCATCA1874940.11744723700385629No Hit
ACGCGAAAGGTCCCCGGTTCGTAACCGGGCGGCTGTAGGCACCATCAATA1863050.11670244109146664No Hit
TTGCATGAGGTCCTGGGTTCGATCCCCAGCATCTCCCTGTAGGCACCATC1795630.11247921649825302No Hit
ACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGCTG1775830.1112389339864519No Hit
AGTTGACCCGGGTTCGTTTCCCGGCCAACGCCTGTAGGCACCATCAATAG1736370.10876713863717556No Hit
ACCGAAAGGTTGGTGGTTCGTGCCCACCCAGGGACCTGTAGGCACCATCA1725310.10807433436543212No Hit
ATCTCAGGGTCGTGGGTTCGGGCCCCACGTTGGGCGCTGTAGGCACCATC1714070.10737025479812685No Hit
TGCGCCGCTGGTGTAGTGGTATCATGCAAGCTGTAGGCACCATCAATAGA1713290.10732139518402561No Hit
AGCAGTTGACCCGGGTTCGATTCCCGGCCAACGCCTGTAGGCACCATCAA1650240.10337190971084079No Hit
ACCCAGAGGTCGATGGATCGGAACCATCCTCTGCCTGTAGGCACCATCAA1631410.10219238851401176No Hit
TTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTAGGCACCATCAATAGAT1625830.10184285435159508No Hit
CCGCCGGGTCCGCCCCCCGGGCCGCGGTCTGTAGGCACCATCAATAGATC1604900.10053178803987807No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)