FastQCFastQC Report
Tue 19 Sept 2023
SRR15618742_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15618742_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160480805
Total Bases8.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA58483823.6442875520221873Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCTCCCCGCGTCCGGCGCTCCGGCGGCGGGCGCGGGGGGCTGTAGGCAC35072582.18546884781641No Hit
ATCTCGGTGGAACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT27988301.7440278916846161No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCAATA22153401.3804392369542264No Hit
TCCCCGTACGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT21537531.3420626846930386No Hit
AACCATCCTCTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC21015331.3095229675598898No Hit
ATCTCGGTGGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT20404711.2714735572269842No Hit
ATCCCACTTCTGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT19810271.2344323671606707No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCAAT18182041.132972881086931No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATAGA16960181.0568354265172089No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG16107381.0036951148145101No Hit
GTGTAATGGTTAGCACTCTGGACTGTAGGCACCATCAATAGATCGGAAGA15738140.9806867556528022No Hit
TTCCTCCCCGCGTCCGGCGCTCCGGCGGCGGGCGCGGGGGGCTGTAGGCA15051220.9378828826288602No Hit
TCGTTTCCCGGTCAGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA13604090.8477082352621549No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGCTGTAGGCACCATCAATAGA12466760.776838077301519No Hit
ATCCCAGCGGTGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT12247090.763149835894704No Hit
TCCCCGTACGGGCCACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC10299870.6418132062585304No Hit
ACCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCA9687670.603665341783399No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA9298220.5793976419796748No Hit
TTCCCGGCCAACGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT8675490.5405936242655314No Hit
ATCCGGGTGCCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT8635840.5381229237976467No Hit
GTGTAATGGTTAGCACTCTGGCTGTAGGCACCATCAATAGATCGGAAGAG8309420.5177827965157578No Hit
TCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGTCTGTAGGCACCATCAATA8188640.5102566627828169No Hit
TCCCCGGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT7459710.46483503120513386No Hit
CCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCAA6671990.41575003315817116No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATAG6564040.4090233719852041No Hit
ATCCGAGTCACGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT6381950.3976768436574081No Hit
TCCCCGTACGGGCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC6335110.3947581145296473No Hit
TCCCCGGCATCTCCACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC5861970.36527546082536166No Hit
GCGCAGCGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATAGATCGGAAG5676510.3537189385359825No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA5638290.35133734529808724No Hit
TTCCCGGTCAGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC5626610.3506095323985943No Hit
ACCCCGTCCGTGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5499700.34270142151891625No Hit
AGCAGTTGACCCGGGTTCGTTTCCCGGCCAACGCCTGTAGGCACCATCAA5355630.3337240238793668No Hit
TCCCGGGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5321030.3315680027901156No Hit
TCGACTCCCGGTGTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA5276530.3287950854932464No Hit
TTGGGGTCGGCGGGGGACCGCCCCCCGGCCGGCGCTGTAGGCACCATCAA5036180.3138182164527402No Hit
TCCCCGTACGGGCCACTGTAGGCACCATCAATAGATCGGAAGAGCACACG4759120.2965538464241876No Hit
TCCCCGTACGGGCCACCCTGTAGGCACCATCAATAGATCGGAAGAGCACA4703360.29307928758208807No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGCTGTAGGCACCATCAATAGAT4694010.29249666338600433No Hit
TGCCCGGATAGCTCAGTCGGTAGAGCATCAGCTGTAGGCACCATCAATAG4545480.2832413508892855No Hit
TCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCCTGTAGGCACCA4477560.27900906902853584No Hit
ACCCCACTCCTGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC4453660.2775197943454982No Hit
TTTCCTCCCCGCGTCCGGCGCTCCGGCGGCGGGCGCGGGGGGCTGTAGGC4176660.2602591630818402No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGCTGTAGGCACCATCAATAGA3980190.2480165774342919No Hit
TCCCCGGCATCTCCACTGTAGGCACCATCAATAGATCGGAAGAGCACACG3912740.24381358256521704No Hit
TGCCCGCATCCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3853370.24011407470195578No Hit
GCGCAATGGATAGCGCATTGGCTGTAGGCACCATCAATAGATCGGAAGAG3824200.23829641183567093No Hit
TTCCCGGGCGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3794720.23645943201743036No Hit
TGCCCGCATCCTCCACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC3738050.23292816857442858No Hit
ATCCCAGCGGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3592750.22387412625453867No Hit
ATCTCGGTGGAACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC3512240.2188573268933939No Hit
TCCGCGCGGCGCCTCGCCTCGGCCGGCGCCCTGTAGGCACCATCAATAGA3459090.21554540432421185No Hit
AACCGGGCGGAAACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3326590.2072889651818484No Hit
ATCTCGCTGGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3235020.20158298682512216No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCCTGTAGGCACCATCAA3223330.20085455079814685No Hit
AGCGCTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT3126520.19482205364062075Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCCGGACGAGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2994540.18659801712734428No Hit
CTCAGTCGGTAGAGCATCAGCTGTAGGCACCATCAATAGATCGGAAGAGC2988350.18621230121571236No Hit
TCGGGCCCCACGTTGGGCGCCACTGTAGGCACCATCAATAGATCGGAAGA2918750.1818753339379124No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG2850450.17761937323282992No Hit
TCGACTCCCGGTATGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA2842470.1771221175018408No Hit
GCCCACCCAGGGACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2792620.1740158270018648No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGTAGGCACCATCAATAG2766820.1724081580971631No Hit
TTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCCTGTAGGCACC2762210.1721208963277571No Hit
GTTGGGAGAGCTTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2676590.16678567882308415No Hit
ATCCGGCTCGAAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2589900.1613837866777899No Hit
AACCGGGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA2569090.160087058386827Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATAGATCGGAAGAGC2556190.15928322393447614No Hit
AGAGCTTGGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG2529100.15759517158453934Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTATAGTGGTGAGTATCCCCGCCCTGTAGGCACCATCAATAGATCGGAAG2472110.15404396806209938No Hit
GCCCACCCAGGGACGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2442480.1521976413316222No Hit
TCCTCACCTGGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2430900.15147605970695374No Hit
ACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGCTGTAGGCACCATCAA2413560.1503955566523984No Hit
ACCCAGAGGTCGATGGATCGTAACCATCCTCTGCCTGTAGGCACCATCAA2411940.15029461000024272No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCCTGTAGGCACCATCAATAG2402430.14970201576443987No Hit
ATCTCGGTGGAACCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2366060.14743570111079637No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAG2330560.14522359854812542No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATA2308070.1438221848401122No Hit
TCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCACTGTAGGCACC2266830.1412524071025192No Hit
GCCTAATGGATAAGGCATTGGCCTGTAGGCACCATCAATAGATCGGAAGA2254640.14049281470142175No Hit
CAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCAAT2252890.14038376739199432No Hit
ATAGCTCAGTCGGTAGAGCATCAGCTGTAGGCACCATCAATAGATCGGAA2218000.13820967560575234No Hit
TGCCCGCATTCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2204370.1373603528471832No Hit
GTGTAATGGTTAGCACTCTGGACCTGTAGGCACCATCAATAGATCGGAAG2175420.13555639878551207No Hit
GCCTCACCTGGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2155160.1342939425060835No Hit
TCCTCCCCGCGTCCGGCGCTCCGGCGGCGGGCGCGGGGGGTGCTGTAGGC2150440.13399982633437063No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGCTGTAGGCACCATCA2139340.13330815482885944No Hit
ACCCCACTTCTGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2126710.13252114481853453No Hit
TGCCCGCATCCTCCACTGTAGGCACCATCAATAGATCGGAAGAGCACACG2059540.1283355975189681No Hit
AGCGCTTTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG2059440.12832936624414365Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGCTGTAGGCACCATCAA2042480.12727254203392113No Hit
GCCTAATGGATAAGGCATCAGCCTGTAGGCACCATCAATAGATCGGAAGA2020200.12588421400303917No Hit
TCCTCACACGGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1973440.12297046989513793No Hit
TGCCCGCATTCTCCACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1933840.12050288506466553No Hit
TTTCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGTCTGTAGGCACCATCAA1912600.1191793622919576No Hit
ATCCCAGTGGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1874080.11677907522958898No Hit
TTCCCCCGCCGGGTCCGCCCCCCGGGCCGCGGTCTGTAGGCACCATCAAT1866180.1162868045184594No Hit
ATCCCATCCTCGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1832360.11417938737283877No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTAGGCACCATCAATAGA1820600.11344658945348636No Hit
TGCGCCGCTGGTGTAGTGGTATCATGCAAGCTGTAGGCACCATCAATAGA1776850.1107204067178003No Hit
TGCCCGCATCCTCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1739750.10840860375793852No Hit
TCGTGCCCCACGTTGGGCGCCACTGTAGGCACCATCAATAGATCGGAAGA1714950.10686324760148105No Hit
AGAGCACTGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1707180.10637907754762323Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCTCGGTGGGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1706060.10630928726958966No Hit
ATCCCTTCGTGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1686230.10507362547190612Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGAAAGGTTGGTGGTTCGTGCCCACCCAGGGACGCTGTAGGCACCATC1679750.10466983886328336No Hit
ATCTCGGTGGAACCTCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1659920.10343417706559985No Hit
ACCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGCTGTAGGCACCATCAA1645820.10255556731535588No Hit
TCCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCA1645620.10254310476570702No Hit
GCCCCACGTTGGGCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1642600.1023549202660094No Hit
ATCCCGGACGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1611070.10039019931386811Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCCGGACGAGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1605880.10006679615048042No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)