FastQCFastQC Report
Wed 28 Jun 2023
SRR1562539.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1562539.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56481495
Total Bases5.7 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATCGACGTATCTCGTATGCCGTCTTCTGCTTGAAA29147155.160477781262695Illumina PCR Primer Index 2 (96% over 30bp)
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATCGACGTA27101594.798313146633247No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATC8959671.5863018498359507No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATCGA4767060.8440038635662884No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATCGACGTATCTCGT4445450.78706309030949No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATC4083930.7230562859570201No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATC3927390.6953410139019869No Hit
ACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATCGACG3927130.6952949811261193No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT3635240.6436161082492594No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA3549890.6285049643250413No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCAA3187490.5643423567311736No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATCGACGT2966830.525274693950647No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATCGACG2597340.45985680796869843No Hit
AAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATCGACGTAT2559670.4531873669420401No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATCGAC2450970.43394212564663875No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATCG2390150.42317399707638753No Hit
ACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATCGACGTATCTC2305230.40813898428148904No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATCGAC2254780.3992068552718019No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGTAGGCACCATCAATC2236750.3960146593145242No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATCGACGT2120830.3754911232431082No Hit
TACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATCGACGTATCT2102660.37227414040651724No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATCG1916610.33933414829051534No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATCG1911660.3384577550576521No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGGACTGTAGGCACCATCA1895130.3355311328073026No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATCGAC1838830.32556326634059524No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATCGAC1793380.317516383020669No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATC1778250.3148376295634526No Hit
ACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATCGACGTATCTCGT1642360.29077842220713174No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1620080.2868337674135573No Hit
TGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATCGACGTAT1556900.275647802877739No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATCGACGTATCTCGT1534220.2716323284289837No Hit
TACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATCG1519570.2690385585579843No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATCGACG1449770.25668052872892266No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC1423570.2520418413145757No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATCG1395160.24701187530535443No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATC1282660.22709384728573492No Hit
ACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATCGACGTATCTCG1273640.22549686406140632No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT1250850.22146191420747627No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT1223260.21657712849137578No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA1163580.2060108359383901No Hit
AAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATCGACGTATCTCG1140570.20193693527411058No Hit
ACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATCGA1109450.19642716610103894No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAATCG1089780.1929446095575197No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATCGA1077250.19072618385897894No Hit
CTGTAGGCACCATCAATCGACGTATCTCGTATGCCGTCTTCTTCTTGAAA1062360.18808992219487108RNA PCR Primer, Index 2 (96% over 26bp)
TAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATCGACGTAT1061400.187919955022437No Hit
TATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATCGACGTATCTCGT1057490.187227692893044No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATCGACGTA1041770.18444448044443584No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATCGA1020310.18064500594398217No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATCGA1002800.17754487553843962No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATCGACG964420.17074972962383522No Hit
AGCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATCGACGTA956000.16925897588227792No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCCTGTAGGCACCATCAAT930370.1647212064765637No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGTAGGCACCATCAATC929570.16457956716620198No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATCGAC915390.16206901039004015No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATCGACG909570.16103858440715849No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATCGACG874780.15487904489780238No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGACTGTAGGCACCATCAA845030.14961183304372522No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATCGACG827190.14645327642265843No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATC818020.144829735827637No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATCGACG774440.1371139343956813No Hit
TGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATCG771780.1366429836887285No Hit
TGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAAT770190.13636147555938452No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGTAGGCACCATCAAT765170.13547268888686465No Hit
CTGTAGGCACCATCAATCGACGTATCTCGTATGCCGTCTTCTCCTTGAAA762820.13505662341267702RNA PCR Primer, Index 2 (96% over 26bp)
CGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATCGACGT760410.13462993499021228No Hit
TTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATCGACG752000.13314095174003449No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATCGAC744910.1318856733519536No Hit
GCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATCGACGTAT722360.12789321529113207No Hit
TCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATCGA711200.12591734691158582No Hit
AAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATCGACGTATCTCGTA686540.12155131516968522No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATCG672010.11897879119524014No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTCTGTAGGCACCATCAATCG666250.11795898816063563No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATC656230.11618495579835485No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATCGAC643920.11400548091016359No Hit
AAAACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATCGACGTA631130.11174102243575529No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATCGA628910.11134797334950146No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATCGA620540.10986607206484177No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATC609760.10795748235771732No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATC590830.10460594217628269No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATC580860.10284076227089953No Hit
TTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGTAGGCACCATCAATCGA578160.10236272959842865No Hit
ACTGTAGGCACCATCAATCGACGTATCTCGTATGCCGTCTTCTGCTTGAA574960.10179617235698171Illumina PCR Primer Index 2 (96% over 30bp)
TATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATCGACGTATCTCG569400.10081177914996761No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)