FastQCFastQC Report
Thu 6 Jul 2023
SRR1562540.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1562540.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78005408
Total Bases7.8 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATTACGATA794675410.187439824684976No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATTACGAT40827535.233935831731051No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATTACGATA13427471.7213511658063503No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT11875791.5224316242278997No Hit
TAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATTACGATAT11068651.4189593111287873No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATTACGAT10101721.295002520850862No Hit
CTAAACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATTACGAT7855971.007105815022466No Hit
ACTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATTACGAT7835491.0044803560286486No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATT7283420.9337070578491172No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATTAC6859410.8793505701553411No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT4803600.6158034581397228No Hit
AAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATTACGATAT4687570.6009288484203557No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTACGA4634620.5941408575159302No Hit
AAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATTACGATATC4067390.5214241043390222No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT3884610.4979923956041612No Hit
TCTAAACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATTACGA3606150.46229487063255925No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATTACGA3230900.4141892315978913No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATT2916270.373854848627931No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT2754360.3530985954204611No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATT2709550.3473541219090861No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATTACGAT2658150.3407648351765559No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATTACGAT2083680.2671199412225368No Hit
TCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATTAC2039020.26139469714715163No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATTACG1895360.2429780253184497No Hit
TTGGCGCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATT1881320.2411781501097975No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTA1847080.23678871085450895No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA1756430.22516772170462848No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTACGA1685290.21604784119583095No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTAC1629070.2088406485868262No Hit
CTGTAGGCACCATCAATTACGATATCTCGTATGCCGTCTTCTGCTTGAAA1495320.19169440149585526RNA PCR Primer, Index 31 (100% over 29bp)
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATTACG1477560.1894176362746542No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATTAC1427400.18298731287964035No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGCTGTAGGCACCATCAATTA1413320.18118230982139086No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATTA1275380.16349892048510278No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTACG1200050.1538418977309881No Hit
TTGGAATGAGTCCACTTTAAATCCTTTAACGACTGTAGGCACCATCAATT1192500.15287401611949777No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATTACGA1168900.14984858485709093No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTA1165960.1494716879116894No Hit
TAAACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATTACGATA1133690.14533479524906787No Hit
CTAAACCATTCGTAGACGACCTGCTCTGTAGGCACCATCAATTACGATAT1125990.14434768420158767No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATTA1125340.14426435664563153No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATTAC1105480.14171837932057224No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATTA1093660.14020309976457018No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT1086340.13926470328826432No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTACGAT1069170.13706357384862342No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATTACG1064640.13648284488172924No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT1056050.1353816391807091No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTAC1055790.13534830815832666No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1051010.13473553013144934No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTA1040970.1334484398825271No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATTACGAT1015800.13022174052342628No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTA979770.12560282999865854No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATT954520.1223658749403631No Hit
TGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATTACGATA926460.11876868844785736No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTCTGTAGGCACCATCAATTACGAT906250.11617784243882169No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT879820.11278961581740589No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATTACGAT877090.11243964008239019No Hit
TTGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT864610.11083975100803269No Hit
CCTAAACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATTACGA847020.10858477914762012No Hit
TCGACTGCATAATTTGTGGTAGTGGGCTGTAGGCACCATCAATTACGATA827830.10612469330331557No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATTAC819260.10502605152709413No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGCTGTAGGCACCATCAATT810650.10392228190127535No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAATTACG810530.10390689835248347No Hit
TGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATTACGATAT802790.10291465945540597No Hit
ATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATTACGATATCTCGTA781630.10020202701843442No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)