FastQCFastQC Report
Sun 2 Jul 2023
SRR1562541.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1562541.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53474607
Total Bases5.4 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATACGACTA23576904.408989859429916No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATACGACTA19028503.5584179234828226No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATACGACT17605583.2923252713198994No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA12426392.3237926741565396No Hit
ACTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATACGACT6030161.1276679415334459No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT5112890.9561341890740778No Hit
TAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATACGACTAT4102200.7671304625015757No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATACG4007730.7494641335092No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT3483200.6513745860722268No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA3432430.6418803601492573No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATACGAC3128210.5849898064701999No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATACGACT2751950.5146274380286703No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATACGACT2635180.49279090541048765No Hit
ATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATACGACTATCTCGTA2364730.44221549865714765No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATACG2205000.41234524640826253No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA2105920.3938168259936908No Hit
TCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATACG2069290.3869668457778474No Hit
CTAAACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATACGACT2031450.37989058993925845No Hit
AAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATACGACTAT2013160.3764702749475092No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATACGAC1954670.3655323731504937No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA1802080.3369973340804543No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAC1690190.3160733841391298No Hit
ACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATACGAC1688540.31576482647174947No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATACGAC1588390.2970363110849978No Hit
AAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATACGACTATC1577270.29495681941150126No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1472460.27535686237020873No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATACGA1408310.2633605142717552No Hit
CTGTAGGCACCATCAATACGACTATCTCGTATGCCGTCTTCTGCTTGAAA1357260.2538139270476546Illumina PCR Primer Index 1 (96% over 27bp)
TCTAAACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATACGAC1317670.2464104130769956No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAC1275670.23855621790731438No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATACGA1191890.2228889685902694No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAC1183410.2213031691845814No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATACGAC1179390.22055141050405475No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATACGACT1159720.2168730290995874No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAC1110540.20767614056518452No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATACG1055160.19731982322001915No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATACGA1030970.19279618081157662No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAC1022810.1912702228928957No Hit
TTGGCGCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATA985670.18432487030713474No Hit
TCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT979100.18309624977702033No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAAT953610.17832950132761144No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATACG930810.17406579537835593No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA904080.16906716116679457No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTCTGTAGGCACCATCAATACGACT886710.1658188904501907No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAATA852190.15936349003930034No Hit
TTGGAATGAGTCCACTTTAAATCCTTTAACGACTGTAGGCACCATCAATA804310.15040970754586377No Hit
TACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAC787780.14731852073265353No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAC776160.1451455267357084No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATA763510.1427799179524592No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATACGACT750080.14026844554463017No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATACGAC737870.13798511880601572No Hit
TCGACTGCATAATTTGTGGTAGTGGGCTGTAGGCACCATCAATACGACTA718180.13430299730861042No Hit
TATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATACGACTATCTCGT715020.13371206262441535No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATACG710600.13288550208512986No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA707670.13233757846972116No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATACGA706670.13215057382282397No Hit
TCGTCGCCGCGCTCTCCCCCCTCCCGGCGCTGTAGGCACCATCAATACGA694070.12979431527191962No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATACG688930.12883311138686815No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT649760.12150813936790597No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATACGA636210.1189742264024493No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATACGA630080.11782788791696963No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA627800.11740151732204408No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAC610710.11420560790657143No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAA586630.10970253600928753No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT581760.10879182337889831No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATACG568700.10634954269042127No Hit
TATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATACGACT546240.10214941832111081No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA542560.1014612412205292No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)