FastQCFastQC Report
Tue 27 Jun 2023
SRR1562542.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1562542.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35287687
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA6858231.9435192791185207No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCAG6265291.7754889970544117No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATATCAGTA6073651.7211811020654315No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATATCAGTA5991991.6980398856972403No Hit
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCAGTA4925561.395829655823007No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCAGT4495271.2738919385676937No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATATC4345391.231418199781697No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA4162561.179606926347992No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT3029630.8585515961984134No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATATCAGT2875400.8148451328079395No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGCTGTAGGCACCATCAATAT2339590.6630046338826345No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATATCA2020860.5726813434952538No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA1925350.5456152453403931No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATATCA1710000.4845882927945943No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCA1609950.4562356268916124No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATATCA1514530.42919503338374093No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATATCAGT1244350.352630083122195No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1229060.3482971269836983No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT1194860.3386053611278064No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1188860.3369050513285271No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATATCAGT1127130.3194116973436088No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGCTGTAGGCACCATCAATA1040350.2948195499466995No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATATC1014500.2874940485614713No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCCCTGTAGGCACCATCAATATC1004360.28462052500068935No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATATCAG980830.27795247673784906No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA966230.2738150562262695No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCAG922320.26137162234521066No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT912140.2584867633857668No Hit
TTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCAGTAT898820.25471207563136683No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAT867650.24587896622411098No Hit
CTGTAGGCACCATCAATATCAGTATCTCGTATGCCGTCTTCTGCTTGAAA859320.24351836945277824RNA PCR Primer, Index 40 (96% over 29bp)
CGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATATCAGTA824310.23359706177398365No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATATCAGTA817040.23153685306719027No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCAG739630.2096000228068221No Hit
TAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATATCAGTAT718520.20361776616302452No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT714500.20247855859750738No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA691700.19601738136024613No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAATATCA678440.19225969670383894No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCAGT677820.19208399802458007No Hit
AAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATATCAGTAT646680.18325939016632062No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATATCAGTA598700.16966257947141733No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATATCAG581230.16471184410584916No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCAGTATCTCGT573690.1625751214580882No Hit
CTAAACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATATCAGT559940.15867857816807318No Hit
TCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATATC550480.15599775638454286No Hit
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCAGTATCTCGT549420.1556973683200035No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATC549300.15566336212401793No Hit
ACTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATATCAGT543820.15411041250734286No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCA528110.1496584346828966No Hit
TAGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATATCAGTA516320.14631732592731284No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATATCAGT514330.1457533898438852No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCCTGTAGGCACCATCAATATCA502860.14250296427759632No Hit
TTGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT483380.1369826251292696No Hit
TATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATATCAGT474290.1344066557833615No Hit
GCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATATCAGTAT473740.13425079405176088No Hit
AGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATATCAGTAT473120.13407509537250203No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAT458010.1297931485279837No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGCTGTAGGCACCATCAATA448890.12720867763307922No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATATC445950.12637552583143236No Hit
TATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATATCAG445650.1262905103414684No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA444170.1258711005909795No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGCTGTAGGCACCATCAATAT442010.12525898906323898No Hit
TCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCAGTATCTC438110.12415378769370744No Hit
CCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATATCA431240.12220693297353266No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAATAT421470.1194382618503729No Hit
TGCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATATCAGTA413750.11725052990863356No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCAGTA405220.11483325614399154No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATATCAGT389610.11040961681619994No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT385160.1091485537150678No Hit
TTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCAGTATC383580.10870080546792427No Hit
TGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATATCAGTAT382480.10838908200472305No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCAGT381230.10803485079653988No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCAG375670.10645923038254108No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATC374560.10614467306967441No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC370550.10500829935382278No Hit
CACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCAGTATCTCG370060.10486944072021495No Hit
TTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCAGTATCT362520.10273271807245399No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT361020.10230764062263417No Hit
AAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATATCAGTATC353980.10031261045814648No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)