FastQCFastQC Report
Thu 29 Jun 2023
SRR1562543.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1562543.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45383508
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATCAGCATA22632334.986906256783852No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATCAG13765763.033207569586732No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATCAGCAT11449602.5228547779955663No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATCAGCATA9697062.1366924742794233No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATCAGC5820181.282443834002431No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATC5778851.2733370016262295No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATCAGCAT5108691.1256710256950608No Hit
ACTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATCAGCAT4972811.0957306341325574No Hit
TAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATCAGCATAT4458530.9824119369529566No Hit
AAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATCAGCATAT3552500.7827733369575574No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATCAGCATA2504620.5518788895737191No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATCAGCAT2171830.4785504901912827No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATCAGC2014780.44394540853915476No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATC1998460.4403493885928783No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA1949080.4294687841230784No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATC1592820.3509689026242749No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATCAGCAT1567870.3454713108559171No Hit
CTAAACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATCAGCAT1543160.3400266017338281No Hit
AAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATCAGCATATC1505890.33181436745700665No Hit
CTGTAGGCACCATCAATCAGCATATCTCGTATGCCGTCTTCTGCTTGAAA1467780.3234170439182445RNA PCR Primer, Index 10 (96% over 29bp)
TATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATCAGCA1388020.30584237780825585No Hit
TGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATCAGCATA1369350.3017285486172642No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATCA1366070.3010058191182577No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATCAGCA1341330.29555449966538505No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATC1330690.2932100356808028No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCCCTGTAGGCACCATCAATCAG1188750.2619343573000131No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAATCAGC1188090.26178893002277387No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1133350.24972727978630474No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATCA1104250.24331525892621614No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATCAGCAT1078920.23773393630126607No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATCAGCA1071990.2362069498902553No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATCA1044890.23023561774907306No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATCAGCA1020440.22484819816044188No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATCAGC1011250.2228232334970668No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAATC966590.21298265440388608No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATCAGCAT926990.20425701776953867No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATCAG901300.19859637117518547No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATCA898950.19807856193047044No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATCA866980.19103415275875105No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATCAGCA857430.1889298641259728No Hit
TACTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATCAGCA836680.18435771866731854No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATC818410.18033202722010824No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATC816050.17981201453179865No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTCTGTAGGCACCATCAATCAGCAT761040.1676908713182771No Hit
ACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATCAGCA725470.15985322245252614No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATCAGC718460.15830860849275907No Hit
TATGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATC707950.15599278927490576No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATCAGCATA691970.15247168641084335No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATC691310.15232625913360423No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT682020.15027926003428382No Hit
TCTAAACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATCAGCA681200.15009857765953216No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATCAGCAT676690.14910482459839816No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATCAGCA657820.14494692653551594No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAAT643050.14169244034639192No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACCA639470.1409036075395494No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATCAG633740.13964103435988245No Hit
TGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATCAGCAT615140.13554262927405258No Hit
TGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAATCA601580.13255475975986697No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATCAGCA599010.13198847475607217No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATCA590520.13011775114431437No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATC581990.12823821375817843No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACCAT571030.12582323957857114No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCCTGTAGGCACCATCAATCAGC569930.1255808607831726No Hit
ACCGGCTCCGGGACGGCTGGGAAGGCCCGCTGTAGGCACCATCAATCAGC558910.12315266594199814No Hit
TTGGCGCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATC556040.12252027763036741No Hit
TGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATCAGCA549520.12108363240673242No Hit
TATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATCAGCAT520400.11466720465945471No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATCAGCAT519730.11451957393862106No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATC518000.11413837819676698No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATCAGCA501620.11052913758892327No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATCA476520.1049984941666475No Hit
TGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATCAGCATAT475900.1048618806637865No Hit
CCGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATCAGCAT456890.10067313439058082No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)