FastQCFastQC Report
Sat 16 Sept 2023
SRR15724857_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15724857_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9590042
Total Bases728.8 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA2033222.1201367001312406No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC1323581.380160795958975No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG890690.9287654840302054No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC769170.8020507105182647No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG602800.6285686757159145No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA590390.6156281693031167No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT555790.5795490780957998No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA383610.40000867566586257No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA377790.3939398805552676No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGG372270.38818390993490953No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGTAGGCACCATCAATAGATCG323480.33730822033938956No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGCTGTAGGCACCATCAATAGA316120.32963359284557875No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACTGTAGGCACCATCAATAG285610.29781934218849093No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCAA276410.28822605782122745No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC268930.280426300531322No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATAGA235340.24540038510780246No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATCAATAGATC226600.23628676495890216No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA223800.23336706971669152Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGG219930.22933163379263616No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGG218060.2273816944701598No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT208190.21708976874136734No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATA202360.21101054614776454No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCA192060.20027023864963261No Hit
TCGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGGCACCATC171520.17885218855141616No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGAAG169900.17716293630413715No Hit
CCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGGAA165480.17255398881464754No Hit
CGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGGCACCATCAATAGAT152260.15876885627821025No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT147310.15360725218930218No Hit
GAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGA147090.1533778475631285No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT142370.14845607558340204No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCTGTAGGCACCATCAATAGATCGGA124880.13021840780259356No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCCTGTAGG123620.1289045449435988No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATC122180.12740298739046188No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATAG117800.12283574983300385No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACTGTAGGCACCATCAATA116470.12144889459295381No Hit
CCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATAGATCGGAAGA107860.11247083172315617No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAAT107330.11191817512373774No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGGCACCATCA106170.1107085870948219No Hit
TTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGAAGAG104450.10891506001746394No Hit
TACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGA104280.10873779280632972No Hit
TGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAAT103430.10785145675065864No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT103030.10743435743034285No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAA100960.10527586844770857No Hit
TACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAG95930.10003084449473736No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)