FastQCFastQC Report
Sat 16 Sept 2023
SRR15724867_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15724867_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13556088
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA2311391.7050567980969142No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC1524031.1242402675462124No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC1303430.961508954500738No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG989690.7300705041159367No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT952130.7023633956935069No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA902980.6661066230906734No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA881630.6503572417057193No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA652580.4813925669411411No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG605580.4467217976159493No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC465900.34368322188525186No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCAA436750.3221799681442021No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATAGA402600.29698833468770636No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGTAGGCACCATCAATAGATCG350940.258879995467719No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGG330650.2439125505824394No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACTGTAGGCACCATCAATAG325010.23975205826341642No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCCTGTAGG312590.23059012305024873No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCA310750.2292327993149646No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATA296460.21869141008822013No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT258100.19039416091131894No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGCTGTAGGCACCATC252200.18604187284709275No Hit
TCGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGGCACCATC232800.17173095955116255No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA232200.17128835398530903No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATCAATAGATC229990.16965809015108194No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT229640.16939990357100074No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC224010.1652467880114086No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGCTGTAGGCACCATCAATAGA214520.15824624331149223No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGG208950.15413738830848545No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCCTGTAG202790.14959330449905606No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT195560.14425990743052125No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA194780.14368452019491168No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA183470.135341405278573No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT179840.13266364160515925No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGG174000.1283556140975184No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCTGTAGGCACCATC170680.1259065299664623No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCCTGTAGGCACCATCA169870.12530901245256007No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTGTGGACTGTAGGCACCATCA168040.12395906547670685No Hit
GAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGA164700.12149522782678897No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATAGATCGG160470.11837485858752171No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA159390.1175781685689854No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT153530.11325538754248275No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCTGTAGGCACCATCAATAGATCGGAA152810.11272426086345855No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT152770.11269475382573499No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATAG152640.11259885595313339No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGGCACCATCA148230.10934570504411006No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGTAGGCACCATCAATAGAT144090.10629172663972083No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT143610.10593764218703804No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCCTGTAGGCACCATCAAT139830.10314922712216092No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA138290.10201320616980356No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA137750.10161486116053539Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAA136270.10052310076476341No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)