FastQCFastQC Report
Sat 16 Sept 2023
SRR15724869_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15724869_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9229927
Total Bases701.4 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA1885202.0424863598596175No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC1277921.3845396610395726No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG798670.8653047851841082No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC764050.8277963628531406No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA575020.622995176451558No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG543320.5886503761080668No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT538570.5835040732174804No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA381620.4134593913906361No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA365570.39607030478139205No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGG342460.37103218692845563No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGTAGGCACCATCAATAGATCG306150.33169276420062693No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACTGTAGGCACCATCAATAG278570.301811704469602No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCAA270450.29301423510716823No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC259420.28106397807913325No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA210380.22793246360453337Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATAGA208640.2260472915982976No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATCAATAGATC207450.22475800729518228No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGG204160.22119351539833412No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGG203560.22054345608583903No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT201080.21785654426085926No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGCTGTAGGCACCATCAATAGA189400.2052020563109546No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCA184960.20039161739849082No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATA179550.19453024926415996No Hit
TCGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGGCACCATC158470.17169149875183196No Hit
CCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGGAA155980.16899375260497726No Hit
CGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGGCACCATCAATAGAT150190.1627206802393995No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGAAG144830.15691348371444325No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT141700.15352234096759376No Hit
GAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGA136000.1473467774988903No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT130300.14117121403018681No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCCTGTAGG121400.1315286675615094No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCTGTAGGCACCATCAATAGATCGGA117680.12749829982403976No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACTGTAGGCACCATCAATA110740.1199792804428464No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATC106520.11540719661163085No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATAG104100.11278529071790058No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT103050.11164768692103415No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGGCACCATCA102960.11155017802415988No Hit
CCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATAGATCGGAAGA101360.10981668652417294No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAAT95810.10380363788359323No Hit
TACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGA94900.10281771459297565No Hit
TTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGAAGAG94180.10203764341798152No Hit
TGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAAT93840.10166927647423431No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)