FastQCFastQC Report
Sat 16 Sept 2023
SRR15724881_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15724881_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8793728
Total Bases668.3 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA1855602.1101403181904193No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC1216151.3829743198788955No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG689850.7844795745331219No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC658870.7492499199429412No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA527980.6004051978864937No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG478660.5443197697267871No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT470080.5345628156795389No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA341130.38792421143797035No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA329300.3744714414637341No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGG309300.3517279588361159No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGTAGGCACCATCAATAGATCG279750.31812446325381No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACTGTAGGCACCATCAATAG248570.2826673738373532No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCAA239220.2720347957089417No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC224750.25557988602785986No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA193990.22060040974658301Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATCAATAGATC191410.21766650048762026No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATAGA189590.215596843568507No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT186950.21259470386166143No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGG183990.2092286684327739No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGCTGTAGGCACCATCAATAGA180710.20549873728184453No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGG177680.20205309966376037No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCA164680.1872698359558085No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATA164430.18698554242296328No Hit
TCGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGGCACCATC143310.16296842476819842No Hit
CCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGGAA140400.15965924804587997No Hit
CGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGGCACCATCAATAGAT138850.15789662814223956No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGAAG128540.14617236284770235No Hit
GAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGA126340.14367057975866435No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT123320.140236313881894No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT121260.13789373517124934No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCCTGTAGG112060.12743173316254494No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCTGTAGGCACCATCAATAGATCGGA105110.1195283729494476No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACTGTAGGCACCATCAATA100740.11455892199531302No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATC95810.10895265352760514No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATAG93210.10599600078601476No Hit
CCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATAGATCGGAAGA93040.10580268118368001No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGGCACCATCA91700.10427886784762957No Hit
TACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGA91100.10359656336880103No Hit
TGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAAT91100.10359656336880103No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT89300.10154964993231538No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAAT88980.1011857542102735No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)