FastQCFastQC Report
Sat 16 Sept 2023
SRR15724903_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15724903_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13534599
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA2317441.7122339568390612No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC1561481.1536950596024307No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC1355771.0017068108187026No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT983790.7268704451458075No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG959270.7087539128421906No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA907920.6708141113009702No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA883340.6526532481678992No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA679950.5023791247897333No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG596640.44082576809257523No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC473230.34964464037685933No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCAA451640.33369293024492264No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATAGA399900.29546497831225No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGTAGGCACCATCAATAGATCG343400.25372011390954397No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACTGTAGGCACCATCAATAG337230.24916142694733698No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGG326850.24149219345176018No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCA319450.23602472448574205No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCCTGTAGG316100.2335495865078825No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATA288600.21323128967470703No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGCTGTAGGCACCATCAATAGA264680.1955580656656322No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGCTGTAGGCACCATC263590.1947527222638809No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT254200.18781494745429844No Hit
TCGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGGCACCATC232190.17155292151618234No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA231720.17120566335212445No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT228710.16898173340783867No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATCAATAGATC224520.1658859638176203No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC220020.16256115160855522No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCCTGTAG211180.15602974273563627No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT203180.15011896547507614No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGG202350.14950572233429302No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA191440.14144489984520414No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT184560.13636163140112242No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA183010.13521641830688888No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCCTGTAGGCACCATCA181840.13435196713253195No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGG177870.131418743916979No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA166690.12315843269534621No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTGTGGACTGTAGGCACCATCA166580.1230771595080135No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCTGTAGGCACCATC166430.12296633243437799No Hit
GAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGA161880.11960457786743442No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATAGATCGG160560.11862929961944199No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCTGTAGGCACCATCAATAGATCGGAA157900.11666396618030576No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT157180.11613199622685533No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATAG148070.10940109862139248No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT147060.10865486299224676No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGGCACCATCA146620.10832977024291596No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT146320.10810811609564495No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCCTGTAGGCACCATCAAT146210.10802684290831224No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGTAGGCACCATCAATAGAT141000.10417744921737246No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA139510.10307656695259314Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA138250.1021456195340549No Hit
CGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGGCACCATCAATAGAT138040.1019904616309652No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAA136760.10104473726927558No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACTGTAGGCACCATCAATA136420.10079352923570177No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)