FastQCFastQC Report
Wed 28 Jun 2023
SRR1583082.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1583082.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22259168
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA11558755.192804151529833Illumina Single End Adapter 1 (95% over 22bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACCTGTAGGCACCATCAATT1877600.8435176013766553No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTCTGTAGGCACCATCAAT1198630.5384882310066575No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC1018150.4574070333626126No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCTGTAGGCACCATCAA942790.42355132051656197No Hit
CGGTGATTGGCGCAGCCTGGTAGCGCACTTCGTTCTGTAGGCACCATCAA871390.39147464990605224No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGCTGTAGGCACCATCAA840460.37757925183906244No Hit
TTCGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGTACCACTGTAGGCACCA683050.3068623229763125No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATC679940.30546514586708723No Hit
GCGGGAGTGGCGAAATTGGTAGACGCACCAGACTGTAGGCACCATCAATT495940.22280257734700595No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCCTGTAGGCACCATCAAT495590.2226453387655819No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCCTGTAGGCACCATCAATT480620.21592002001152963No Hit
GCCGATATAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATTC463040.20802215069314361No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA450930.20258169577587087Illumina Single End Adapter 1 (95% over 22bp)
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCAT439200.19731195703271567No Hit
TATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGTAGGCAC435710.19574406374937284No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCCTGTAGGCACCATCAAT427950.19225785977265636No Hit
TCGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGTACCACTGTAGGCACCAT427460.19203772575866268No Hit
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGTACCACTGTAGGCACCATCA415050.18646249491445505No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCCTGTAGGCACCATCAATTCGTA368490.1655452710541562No Hit
TGCCGACTTAGCTCAGTAGGTAGAGCAACTGACTGTAGGCACCATCAATT354430.1592287726118065No Hit
TATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCACTGTAGGCAC341750.15353224343335742No Hit
TTAGGTTAATGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATTC340150.15281343848970458No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATCA339720.15262025966109785No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCATCAATTC335460.15070644149862206No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTGTAGGCACCATCAATTC330360.14841525074072848No Hit
ATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGTAGGCACC329260.14792107234196714No Hit
AGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGCTGTAGGCACCA327720.14722922258370125No Hit
CGGTGATTGGCGCAGCCTGGTAGCGCACTTCGTCTGTAGGCACCATCAAT317530.14265133359881196No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG316830.1423368564359638No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCTCTGTAGGCACCATCAATTC294140.13214330382878642No Hit
TTGGGGTATCGCCAAGCGGTAAGGCACCGGACTGTAGGCACCATCAATTC259920.11676986309641044No Hit
TGCCGATATAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATT259670.11665754982396467No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG249410.11204821312279058Illumina Single End Adapter 1 (95% over 22bp)
TTGGGGTATCGCCAAGCGGTAAGGCACCGGTCTGTAGGCACCATCAATTC244130.10967615680873607No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATTCGT223240.10029125976316815No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)