FastQCFastQC Report
Wed 28 Jun 2023
SRR1583083.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1583083.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26096770
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA12076694.627656985902854Illumina Single End Adapter 1 (95% over 22bp)
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG3823921.4652847842855647No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATC1790350.6860427554827666No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT1678300.6431064074213015Illumina Single End Adapter 1 (95% over 22bp)
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTCTGTAGGCACCATCAAT1509040.5782478061461246No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACCTGTAGGCACCATCAATT1341030.5138681913508836No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCCTGTAGGCACCATCAAT1340220.5135578081118851No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCAT867330.3323514749143285No Hit
ACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATTCGTAT842280.3227525858564106No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC821180.31466729407509053Illumina Single End Adapter 1 (95% over 22bp)
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCTGTAGGCACCATCAA803720.3079768109233441No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG790210.30279992504819564Illumina Single End Adapter 1 (95% over 22bp)
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA786590.3014127802023009Illumina Single End Adapter 1 (95% over 22bp)
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA761160.2916682792544824No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCCTGTAGGCACCATCAATT714080.2736277324741721No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATCA597280.2288712357889501No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA536750.20567679448452816Illumina Single End Adapter 1 (95% over 22bp)
TAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCCTGTAGGCACCATCAA484370.18560534502928908No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCCTGTAGGCACCATCAATTCGTA484100.1855018839496229No Hit
AGTAGGTAGCTTAACCTTCGGGAGGGCGCCTGTAGGCACCATCAATTCGT440150.16866071931507232No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCTCTGTAGGCACCATCAATT435760.166978518797537No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGCTGTAGGCACCATCAA435570.16690571285258674No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA423280.16219631778185575Illumina Single End Adapter 1 (95% over 22bp)
GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCCTGTAGGCACCATCAAT418470.16035317780706196No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCATCAATTC417470.15996998862311312No Hit
AAGGCTGAGGCGTGATGACGAGGCACTCTGTAGGCACCATCAATTCGTAT412120.15791992648898695No Hit
AGCGCACGCAGGCGGTTTGTTAAGTCAGATGCTGTAGGCACCATCAATTC408870.1566745616411533No Hit
TTACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATTCGT406260.15567443787104687No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT391680.15008753956907311No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC376030.1440906288402741No Hit
ACGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATTCGTATG358230.13726986136598512No Hit
TACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATTCGTA348940.13371003384710062No Hit
ACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATCAATT347810.13327703006923844No Hit
AGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCTGTAGGCACCATCAAT330870.12678580529314548No Hit
AGTAGGTAGCTTAACCTTCGGGAGGGCGCTCTGTAGGCACCATCAATTCG328930.12604241827628476No Hit
GCCGATATAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATTC324980.12452882099968693No Hit
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGTACCACTGTAGGCACCATCA322420.12354785668877796No Hit
AAGTAGGTAGCTTAACCTTCGGGAGGGCTGTAGGCACCATCAATTCGTAT313880.12027542105785506No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATC313060.11996120592701702Illumina Single End Adapter 1 (95% over 22bp)
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAACAA312040.11957035295938921Illumina Single End Adapter 1 (95% over 22bp)
ACGTATCGGGGGTTCGAATCCCCCCCTCACCGCCACTGTAGGCACCATCA310950.11915267674888501No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTGTAGGCACCATCAATTC300540.11516367734397781No Hit
ACTACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATTCG298100.1142286957351427No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGA295090.11307529629145675Illumina Single End Adapter 1 (95% over 22bp)
CGGTGGTTCAAGTCCACTCAGGCCTCTGTAGGCACCATCAATTCGTATGC285290.10932004228875833No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTCG282390.10820879365530678No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA279550.10712053637289214Illumina Single End Adapter 1 (95% over 22bp)
CGGTGATTGGCGCAGCCTGGTAGCGCACTTCGTTCTGTAGGCACCATCAA276090.10579470179642922No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT275350.10551114180030709No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCTTCTGTAGGCACCATCAAT269480.10326182129052754No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)