FastQCFastQC Report
Wed 28 Jun 2023
SRR1583084.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1583084.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23465502
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA10646374.537030573648073Illumina Single End Adapter 1 (95% over 22bp)
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG3204241.3655109530578124No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATC1940050.8267668852769482No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT1490470.6351749900769222Illumina Single End Adapter 1 (95% over 22bp)
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTCTGTAGGCACCATCAAT1080080.4602842078554296No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCCTGTAGGCACCATCAAT1050470.4476656838622076No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCAT894420.3811638037831025No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC742670.31649440101473214Illumina Single End Adapter 1 (95% over 22bp)
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCCTGTAGGCACCATCAATT734040.312816661667839No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG713920.3042423724836571Illumina Single End Adapter 1 (95% over 22bp)
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA698790.29779460929495566No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACCTGTAGGCACCATCAATT665790.283731411328852No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATCA563170.23999912722941105No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA476370.20300865500341736Illumina Single End Adapter 1 (95% over 22bp)
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCTGTAGGCACCATCAA461860.19682510947347304No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCCTGTAGGCACCATCAATTCGTA454350.19362466654239913No Hit
CGGCACGTAGCGCAGCCTGGTAGCGCACCGCTGTAGGCACCATCAATTCG428700.18269372630510952No Hit
AAGTTTGAACGTACAAAACCGCACGTTCTGTAGGCACCATCAATTCGTAT384650.16392148780793184No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA379490.16172251503505017Illumina Single End Adapter 1 (95% over 22bp)
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA378050.1611088482147111Illumina Single End Adapter 1 (95% over 22bp)
AGCGCACGCAGGCGGTTTGTTAAGTCAGATGCTGTAGGCACCATCAATTC365370.15570517093561434No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCTCTGTAGGCACCATCAATT339230.14456541351640378No Hit
CGGTGGTTCAAGTCCACTCAGGCCTCTGTAGGCACCATCAATTCGTATGC327950.1397583567570811No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA308860.13162300981244723No Hit
ACCTGCGTGAGCGTACTGGTAAGGCTGCTGTAGGCACCATCAATTCGTAT301210.12836290482939594No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT300110.12789413156385915No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC300070.12787708526329417No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCATCAATTC293940.1252647397017119No Hit
ACCAAAGAATCCGTATCCGCCTGAAAGCGCTGTAGGCACCATCAATTCGT293470.12506444567007344No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTCG281860.12011675693108974No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATTC280440.11951161326103316No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATC277780.11837803427346238Illumina Single End Adapter 1 (95% over 22bp)
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAACAA275010.11719757795933793Illumina Single End Adapter 1 (95% over 22bp)
AACTGGCTCGTCGCCTGCGCGGTAAGCACACTGTAGGCACCATCAATTCG266590.11360933169041088No Hit
ACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATCAATT262980.11207090306442197No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGA261850.11158934507346145Illumina Single End Adapter 1 (95% over 22bp)
ACGCGGTGCTGCTAAGCGCTTCAAAAAAACCGGTCTGTAGGCACCATCAA260470.11100124770396987No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGTA244070.10401226447233047No Hit
AGTAGGTAGCTTAACCTTCGGGAGGGCGCCTGTAGGCACCATCAATTCGT239310.10198375470509857No Hit
ACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCTGTAGGCACCA235690.10044106450396843No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)