FastQCFastQC Report
Wed 28 Jun 2023
SRR1585486.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585486.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24004434
Total Bases686 Mbp
Sequences flagged as poor quality0
Sequence length21-48
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTTGGGCTGCAGGTCGACCCGTGC2353000.9802355681454519No Hit
CGGGGGAAGGTGGCTCGGGGGGC1245070.5186833399196165No Hit
CTGGTCCGAAGGTAGTGAGTTATCTCAA495020.20622023414507504No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAA471680.19649703050694717No Hit
GATCAAAAGGGACAAGCATCAAGCACGCAAC461140.19210617505082603No Hit
AATACAAGCTTGGGCTGCAGGTCGACCCG453820.18905673843424092No Hit
AAACGCTGCCGCCCGGCGATGCGGCGGCG446480.18599897002362148No Hit
TCGACTGCATCCTTTGTGGTAGTGGGGG446170.1858698272160885No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAA424480.17683399658579743No Hit
TCGACTGCATACTTTGTGGTCGTGGGGG421530.17560505696572556No Hit
AATACAAGCTTGGGCTGCAGGTCGAC415940.17327632053311484No Hit
GGAGAGCGACGCTCAGACAGGCGT378490.15767503620372803No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAA358290.1492599242289987No Hit
CGGGGGAAGGTGGCTCGGGGGG341630.1423195397983556No Hit
TCGACTGCATCATTTGTGGTCGTGGGGG336100.140015798747848No Hit
CGACTGCCTACTTTGTGGTAGTGGGGG258910.10785923967213723No Hit
GGAGAGATCCTTCCGCAGGTTCACC257780.10738849330919446No Hit
GGCCCGGCGGGGGAAGGTGGCTCGGGGGGC254180.10588877038300508No Hit
AAAGCACCCAACTTACACTTAGGAGATT241620.100656403729411No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)