FastQCFastQC Report
Wed 28 Jun 2023
SRR1585487_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585487_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18542777
Total Bases530.1 Mbp
Sequences flagged as poor quality0
Sequence length21-42
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATACAAGCTTGGGCTGCAGGTCGACCCG1072690.5784947961138722No Hit
CGGGGGAAGGTGGCTCGGGGGGC717670.3870348006665884No Hit
GGAGAGCGACGCTCAGACAGG523790.28247656756051154No Hit
AATACAAGCTTGGGCTGCAGGTCGAC504470.27205741621117485No Hit
GGAGAGCGACGCTCAGACAGGC412620.2225233038179772No Hit
GATCAAAAGGGACAAGCATCAAGCACGCAAC403380.2175402314335118No Hit
CCTCTAGCATCACCAGTATTAGAGGC401480.2165155736921174No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTT374170.2017874668934432No Hit
AATACAAGCTTGGGCTGCAGGTCG337450.1819846078071262No Hit
ACCTCTAGCATCACCAGTATTAGAGGC304770.16436049465514255No Hit
CGGGGGAAGGTGGCTCGGGGGG285740.1540977384347555No Hit
AATACAAGCTTGGGCTGCAGGTCGACC248350.13393355267120993No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAA237620.1281469329000721No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT216900.11697277058339212No Hit
AAAGCACCCAACTTACACTTAGGAGATT204720.11040417516750593No Hit
AATACAAGCTTGGGCTGCAGGTCGACCCGTG202060.10896965432955377No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)