FastQCFastQC Report
Wed 28 Jun 2023
SRR1585499.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585499.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13893422
Total Bases397.3 Mbp
Sequences flagged as poor quality0
Sequence length21-48
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGCCGGGCCCCCCCTCCCCCGGCG680860.4900592525009318No Hit
GGAGAGCGACGCTCAGACAGG442350.31838808322384504No Hit
TGGGGGGGCCGGGCCCCCCCTCCCCCGGCGC399200.28733022001347114No Hit
GGAGAGCGACGCTCAGACAGGC331020.2382566368458397No Hit
CCTCTAGCATCACCAGTATTAGAGGC289970.2087102803038733No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTT283810.20427652740987787No Hit
GGTCGGTTTCTATCTACTTCAAATTCCT243050.17493890274116772No Hit
AATACAAGCTTGGGCTGCAGGTCGACCCG228570.16451670437995766No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACC222830.16038525281964372No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACC217900.15683681097428695No Hit
ACCTCTAGCATCACCAGTATTAGAGGC197070.14184410435384456No Hit
TTGGGGGGCCGGGCCCCCCCTCCCCCGGCG190840.13735996790423555No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTC168440.12123723010788846No Hit
GGTCGGTTTCTATCTACTTCAAATTCCTC168320.12115085829826518No Hit
ACTATGCCGACCTGCGATGAGGCGGCT167170.12032312845604201No Hit
ACGATTAAAGTCCTACGTGATCTGAGTT163470.11765999765932396No Hit
GATCAAAAGGGACAAGCATCAAGCACGCAAC163090.11738648692885022No Hit
CGATTAAAGTCCTACGTGATCTGAGTTC161490.11623486280053971No Hit
AATACAAGCTTGGGCTGCAGGTCGAC148740.10705785802806536No Hit
AAACGCTGCCGCCCGGCGATGCGGCGGCG139780.10060876290952654No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)