FastQCFastQC Report
Wed 28 Jun 2023
SRR1585504.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585504.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24822786
Total Bases710.5 Mbp
Sequences flagged as poor quality0
Sequence length21-48
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTTGGGCTGCAGGTCGACCCGTGC1974950.7956197986801321No Hit
AAACGCTGCCGCCCGGCGATGCGGCGGCG1179040.47498294510535605No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAA857510.3454527626351047No Hit
CTGGTCCGAAGGTAGTGAGTTATCTCAA476550.19198086790096808No Hit
TGGTCCGATGGTAGTGGGTTATCAGAA416580.16782161357713837No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAA373800.15058744816153996No Hit
AATACAAGCTTGGGCTGCAGGTCGAC324530.13073874946994266No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACC321180.129389182986954No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAA314860.12684313517427093No Hit
CCTCTAGCATCACCAGTATTAGAGGC308900.12444211540155081No Hit
AATACAAGCTTGGGCTGCAGGTCGACCCG301830.1215939258389449No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTT288920.11639305918360655No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACC276450.11136944902155625No Hit
AAACGCTGCCGCCCGGCGATGCGGCGGCGTT265140.10681315143271992No Hit
GGTACACGGAGTCAAGACCCGCAACGCGG258250.1040374758900955No Hit
ACCTCTAGCATCACCAGTATTAGAGGC252760.10182579828066035No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)