FastQCFastQC Report
Wed 28 Jun 2023
SRR1585514_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585514_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28003170
Total Bases789.3 Mbp
Sequences flagged as poor quality0
Sequence length21-42
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATACAAGCTTGGGCTGCAGGTCGACCCG1875920.6698955868210634No Hit
GGAGAGCGACGCTCAGACAGGC1467520.524054955206857No Hit
GGAGAGCGACGCTCAGACAGG837500.29907328348897644No Hit
TGGGGGGGCCGGGCCCCCCCTCCCCCGGCG836200.2986090503325159No Hit
AATACAAGCTTGGGCTGCAGGTCGAC705100.25179292201561465No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTT677250.24184761939451854No Hit
CCTCTAGCATCACCAGTATTAGAGGC609180.21753965711739065No Hit
TGGGGGGGCCGGGCCCCCCCTCCCCCGGCGC608450.21727897234491667No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACC587480.2097905344287807No Hit
AATACAAGCTTGGGCTGCAGGTCGACC485450.17335537369519236No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACC476060.17000218189583538No Hit
AATACAAGCTTGGGCTGCAGGTCGACCC447390.15976405528374107No Hit
GGTACACGGAGTCAAGACCCGCAACGCGG355720.12702847570471484No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT347250.12400381813916067No Hit
GGTCGGTTTCTATCTACTTCAAATTCCT324910.11602614989660098No Hit
CTCTAGCATCACCAGTATTAGAGGC322480.11515839099644791No Hit
ACCTCTAGCATCACCAGTATTAGAGGC313570.11197660836255324No Hit
CTGGTCCGAAGGTAGTGAGTTATCTCAA300730.10739141318643566No Hit
AAAGCACCCAACTTACACTTAGGAGATT298420.10656650657764816No Hit
GGTACACGGAGTCAAGACCCGCAACGCGT294470.10515595198686434No Hit
GGAGAGCGACGCTCAGACAGGCG292560.10447388634929546No Hit

[OK]Adapter Content

Adapter graph