FastQCFastQC Report
Wed 28 Jun 2023
SRR1585515_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585515_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27691077
Total Bases767.3 Mbp
Sequences flagged as poor quality0
Sequence length21-42
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCTGGGAATACCGGGTGCTGTAGGCTT5780312.087426935398721No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT2813941.0161901611844133No Hit
AATACAAGCTTGGGCTGCAGGTCGACCCG1593140.5753261240073834No Hit
CCTCTAGCATCACCAGTATTAGAGGC1177130.4250936140909218No Hit
CTCTAGCATCACCAGTATTAGAGGC869730.3140831250442155No Hit
GGAGAGCGACGCTCAGACAGG737930.26648656532932974No Hit
AAAGCACCCAACTTACACTTAGGAGATT707850.25562386035039375No Hit
AATACAAGCTTGGGCTGCAGGTCGAC627230.22650978869474814No Hit
GGAGAGCGACGCTCAGACAGGC574910.2076156156728754No Hit
CGCCTGGGAATACCGGGTGCTGTAGGCTT537490.19410223733804213No Hit
AGCTTGGGCTGCAGGTCGACCCG522930.18884422588547206No Hit
AATACAAGCTTGGGCTGCAGGTCG498140.17989188358401517No Hit
GATCAAAAGGGACAAGCATCAAGCACGCAGC474050.17119233029470107No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTT470490.16990671760437487No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACC428140.15461298236973592No Hit
ACCTCTAGCATCACCAGTATTAGAGGC379920.1371994307046996No Hit
GGTCGGTTTCTATCTACTTCAAATTCCT322930.11661879384467422No Hit
AGGGACAAGCATCAAGCACGCAGC316080.11414507279727688No Hit
AATACAAGCTTGGGCTGCAGGTCGACC315460.11392117395795041No Hit
GGGACAAGCATCAAGCACGCAGC300230.10842120730804367No Hit
AATACAAGCTTGGGCTGCAGGTCGACCCGTG298970.10796618708618666No Hit
CAAAGCACCCAACTTACACTTAGGAGATT298910.1079445194565744No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACC298540.10781090240729893No Hit
GCCTGGGAATACCGGGTGCTGTAGGCTT292250.10553941256961584No Hit

[OK]Adapter Content

Adapter graph